Browsing by Author "Weller, Peter"
Now showing items 21-40 of 50
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Functional extracellular eosinophil granules: Novel implications in eosinophil immunobiology
Neves, Josiane S; Weller, Peter Fahey (Elsevier BV, 2009)Human eosinophils contain within their cytoplasmic granules multiple preformed proteins, including over three dozen cytokines with nominal Th1, Th2 and immunoregulatory capabilities, and four distinctive cationic proteins. ... -
A Gel-Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion: EliCell
Spencer, Lisa Ann; Melo, Rossana; Perez, Sandra A. C.; Weller, Peter Fahey (2005)A distinguishing feature of eosinophils is their ability to rapidly release preformed cytokines from intracellular pools. Cytokines are delivered to the cell surface from granule stores by transport vesicles and are released ... -
Human eosinophil leukocytes express protein disulfide isomerase in secretory granules and vesicles: Ultrastructural studies
Dias, F. F.; Amaral, K. B.; Carmo, L. A. S.; Shamri, R.; Dvorak, Ann Marie; Weller, Peter Fahey; Melo, Rossana (Journal of Histochemistry & Cytochemistry (The Histochemical Society), 2014)Protein disulfide isomerase (PDI) has fundamental roles in the oxidative folding of proteins in the endoplasmic reticulum (ER) of eukaryotic cells. The study of this molecule has been attracting considerable attention due ... -
Human Eosinophils Secrete Preformed, Granule-Stored Interleukin-4 Through Distinct Vesicular Compartments
Melo, Rossana C. N.; Spencer, Lisa A.; Perez, Sandra A. C.; Ghiran, Ionita; Dvorak, Ann M.; Weller, Peter Fahey (Wiley-Blackwell, 2005)Secretion of interleukin-4 (IL-4) by leukocytes is important for varied immune responses including allergic inflammation. Within eosinophils, unlike lymphocytes, IL-4 is stored in granules (termed specific granules) and ... -
Hypereosinophilic syndromes: A multicenter, retrospective analysis of clinical characteristics and response to therapy
Ogbogu, Princess U.; Bochner, Bruce S.; Butterfield, Joseph H.; Gleich, Gerald J.; Huss-Marp, Johannes; Kahn, Jean Emmanuel; Leiferman, Kristin M.; Nutman, Thomas B.; Pfab, Florian; Ring, Johannes; Rothenberg, Marc E.; Roufosse, Florence; Sajous, Marie-Helene; Sheikh, Javed; Simon, Dagmar; Simon, Hans-Uwe; Stein, Miguel L.; Wardlaw, Andrew; Weller, Peter Fahey; Klion, Amy D. (Elsevier BV, 2009)Background: Hypereosinophilic syndrome (HES) is a heterogeneous group of rare disorders defined by persistent blood eosinophilia ≥1.5 × 109/L, absence of a secondary cause, and evidence of eosinophil-associated pathology. ... -
Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays
Bandeira-Melo, Christianne; Weller, Peter Fahey; Bozza, Patricia T. (Springer Science + Business Media, 2011)Eicosanoids, arachidonic acid-derived signaling lipid mediators, are newly formed and nonstorable molecules that have important roles in physiological and pathological processes. EicosaCell is a microscopic assay that ... -
IL-16 Promotes Leukotriene C4 and IL-4 Release from Human Eosinophils via CD4- and Autocrine CCR3-Chemokine-Mediated Signaling
Bandeira-Melo, C.; Sugiyama, K.; Woods, L. J.; Phoofolo, M.; Center, D. M.; Cruikshank, W. W.; Weller, Peter (The American Association of Immunologists, 2002)Human eosinophils are potential sources of inflammatory and immunomodulatory mediators, including cysteinyl leukotrienes, chemokines, and cytokines, which are pertinent to allergic inflammation. We evaluated the means by ... -
Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
Melo, Rossana C.N.; D’Ávila, Heloisa; Bozza, Patricia T.; Weller, Peter Fahey (Springer Science + Business Media, 2010)Lipid bodies, also known as lipid droplets, are present in most eukaryotic cells. In leukocytes, lipid bodies are functionally active organelles with central roles in inflammation and are considered structural markers of ... -
Imported Fasciola hepatica Infection in the United States and Treatment with Triclabendazole
Graham, Camilla Suzanne; Brodie, Sharon B.; Weller, Peter Fahey (Oxford University Press (OUP), 2001)Infection with Fasciola hepatica, a liver trematode, is not frequently reported in the United States. We describe 2 patients, both originally from Cape Verde, who illustrate the spectrum of clinical presentations of F. ... -
The Internal Architecture of Leukocyte Lipid Body Organelles Captured by Three-Dimensional Electron Microscopy Tomography
Melo, Rossana C. N.; Paganoti, Guillherme F.; Dvorak, Ann Marie; Weller, Peter Fahey (Public Library of Science, 2013)Lipid bodies (LBs), also known as lipid droplets, are complex organelles of all eukaryotic cells linked to a variety of biological functions as well as to the development of human diseases. In cells from the immune system, ... -
Intragranular Vesiculotubular Compartments are Involved in Piecemeal Degranulation by Activated Human Eosinophils
Melo, Rossana C.N.; Perez, Sandra A.C.; Spencer, Lisa A.; Dvorak, Ann M.; Weller, Peter Fahey (Wiley-Blackwell, 2005)Eosinophils, leukocytes involved in allergic, inflammatory and immunoregulatory responses, have a distinct capacity to rapidly secrete preformed granule-stored proteins through piecemeal degranulation (PMD), a secretion ... -
Leukocyte lipid bodies — Biogenesis and functions in inflammation
Bozza, Patricia T.; Magalhães, Kelly G.; Weller, Peter Fahey (Elsevier BV, 2009)Lipid body accumulation within leukocytes is a common feature in both clinical and experimental infectious, neoplasic and other inflammatory conditions. Here, we will review the contemporary evidence related to the biogenesis ... -
Leukocyte lipid bodies: inflammation-related organelles are rapidly detected by wet scanning electron microscopy
Melo, R. C. N.; Sabban, A.; Weller, Peter Fahey (American Society for Biochemistry & Molecular Biology (ASBMB), 2006)Leukocyte lipid bodies are dynamic, functionally active organelles with central roles in inflammation. Here, we report that leukocyte lipid bodies are facilely detected by a versatile, potent technique, termed wet scanning ... -
Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins
Melo, R. C. N.; Spencer, L. A.; Dvorak, A. M.; Weller, Peter Fahey (Society for Leukocyte Biology, 2007)Eosinophils generate and store a battery of proteins, including classical cationic proteins, cytokines, chemokines, and growth factors. Rapid secretion of these active mediators by eosinophils is central to a range of ... -
Molecular and Morphological Characterization of Piecemeal Degranulation in Human Neutrophil Azurophilic Granules
Mahmudi-Azer, Salahaddin; Weller, Peter Fahey; Dvorak, Ann Marie; Moqbel, Redwan; Paré, Peter D (BioMed Central, 2010) -
Novel targeted therapies for eosinophilic disorders
Wechsler, Michael E.; Fulkerson, Patricia C.; Bochner, Bruce S.; Gauvreau, Gail M.; Gleich, Gerald J.; Henkel, Tim; Kolbeck, Roland; Mathur, Sameer K.; Ortega, Hector; Patel, Jatin; Prussin, Calman; Renzi, Paolo; Rothenberg, Marc E.; Roufosse, Florence; Simon, Dagmar; Simon, Hans-Uwe; Wardlaw, Andrew; Weller, Peter Fahey; Klion, Amy D. (Elsevier BV, 2012)Hypereosinophilic syndromes (HESs) are a diverse group of conditions characterized by clinical manifestations attributable to eosinophilia and eosinophilic infiltration of tissues. HESs are chronic disorders with significant ... -
Pathogenesis and classification of eosinophil disorders: a review of recent developments in the field
Valent, Peter; Gleich, Gerald J; Reiter, Andreas; Roufosse, Florence; Weller, Peter Fahey; Hellmann, Andrzej; Metzgeroth, Georgia; Leiferman, Kristin M; Arock, Michel; Sotlar, Karl; Butterfield, Joseph H; Cerny-Reiterer, Sabine; Mayerhofer, Matthias; Vandenberghe, Peter; Haferlach, Torsten; Bochner, Bruce S; Gotlib, Jason; Horny, Hans-Peter; Simon, Hans-Uwe; Klion, Amy D (Informa Healthcare, 2012)Eosinophils and their products play an essential role in the pathogenesis of various reactive and neoplastic disorders. Depending on the underlying disease, molecular defect and involved cytokines, hypereosinophilia may ... -
Pivotal Advance: Eosinophils mediate early alum adjuvant-elicited B cell priming and IgM production
Wang, H.-B.; Weller, Peter Fahey (Society for Leukocyte Biology, 2008)Alum, aluminum-hydroxide-containing compounds, long used as adjuvants in human vaccinations, functions by ill-defined, immunostimulatory mechanisms. Antigen-free alum has been shown to act via a previously unidentified, ... -
Practical approach to the patient with hypereosinophilia
Roufosse, Florence; Weller, Peter Fahey (Elsevier BV, 2010)Markedly increased blood eosinophilia (ie, ≥1.5 × 109/L), whether discovered fortuitously or found with signs and symptoms of associated organ involvement, commands diagnostic evaluation and often therapeutic interventions. ... -
Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes
Melo, Rossana; Morgan, Ellen; Monahan-Earley, Rita; Dvorak, Ann Marie; Weller, Peter Fahey (Nature Publishing Group, 2014)Precise immunolocalization of proteins within a cell is central to understanding cell processes and functions such as intracellular trafficking and secretion of molecules during immune responses. Here we describe a protocol ...