Browsing by Author "Orkin, Stuart"

Now showing items 21-33 of 33

    • The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability 

      Chung, Jacky; Bauer, Daniel Evan; Ghamari, Alireza; Nizzi, C. P.; Deck, K. M.; Kingsley, P. D.; Yien, Yvette Yee; Huston, N. C.; Chen, C.; Schultz, I. J.; Dalton, Arthur J.; Wittig, Johannes; Palis, J.; Orkin, Stuart Holland; Lodish, H. F.; Eisenstein, R. S.; Cantor, Alan B.; Paw, Barry Htin (American Association for the Advancement of Science (AAAS), 2015)
      In multicellular organisms, the mechanisms by which diverse cell types acquire distinct amino acids and how cellular function adapts to their availability are fundamental questions in biology. We found that increased neutral ...

    • Networking Erythropoiesis 

      Kerenyi, Marc; Orkin, Stuart Holland (The Rockefeller University Press, 2010)
      A relatively small cadre of lineage-restricted transcription factors largely orchestrates erythropoiesis, but how these nuclear factors interact to regulate this complex biology is still largely unknown. However, recent ...

    • Rb and Hematopoiesis: Stem Cells to Anemia 

      Walkley, Carl R; Sankaran, Vijay Ganesh; Orkin, Stuart Holland (BioMed Central, 2008)
      The retinoblastoma protein, Rb, was one of the first tumor suppressor genes identified as a result of the familial syndrome retinoblastoma. In the period since its identification and cloning a large number of studies have ...

    • Scl binds to primed enhancers in mesoderm to regulate hematopoietic and cardiac fate divergence 

      Org, Tõnis; Duan, Dan; Ferrari, Roberto; Montel-Hagen, Amelie; Van Handel, Ben; Kerényi, Marc A; Sasidharan, Rajkumar; Rubbi, Liudmilla; Fujiwara, Yuko; Pellegrini, Matteo; Orkin, Stuart H; Kurdistani, Siavash K; Mikkola, Hanna KA (BlackWell Publishing Ltd, 2015)
      Scl/Tal1 confers hemogenic competence and prevents ectopic cardiomyogenesis in embryonic endothelium by unknown mechanisms. We discovered that Scl binds to hematopoietic and cardiac enhancers that become epigenetically ...

    • A Single cis Element Maintains Repression of the Key Developmental Regulator Gata2 

      Snow, Jonathan W.; Trowbridge, Jennifer J.; Fujiwara, Tohru; Emambokus, Nikla E.; Grass, Jeffrey A.; Orkin, Stuart Holland; Bresnick, Emery H. (Public Library of Science, 2010)
      In development, lineage-restricted transcription factors simultaneously promote differentiation while repressing alternative fates. Molecular dissection of this process has been challenging as transcription factor loci are ...

    • Single-Cell Transcript Profiles Reveal Multilineage Priming in Early Progenitors Derived from Lgr5 + Intestinal Stem Cells 

      Kim, Tae-Hee; Saadatpour, Assieh; Guo, Guoji; Saxena, Madhurima; Cavazza, Alessia; Desai, Niyati; Jadhav, Unmesh Jaysing; Jiang, Lan; Rivera, Miguel Nicolas; Orkin, Stuart Holland; Yuan, Guo-Cheng; Shivdasani, Ramesh Arjun (Elsevier BV, 2016-08)
      Lgr5+ intestinal stem cells (ISC) drive epithelial self-renewal, and their immediate progeny – intestinal bipotential progenitors – produce absorptive and secretory lineages via lateral inhibition. To define features of ...

    • Sox2 maintains self-renewal of tumor initiating cells in osteosarcomas 

      Basu-Roy, Upal; Seo, Eunjeong; Ramanathapuram, Lalitha; Rapp, Timothy B.; Perry, Jennifer; Orkin, Stuart Holland; Mansukhani, Alka; Basilico, Claudio (2011)
      Tumors are thought to be sustained by a reservoir of self-renewing cells, termed tumor initiating cells or cancer stem cells. Osteosarcomas are high-grade sarcomas derived from osteoblast progenitor cells and are the most ...

    • Targeted Disruption of the EZH2/EED Complex Inhibits EZH2-dependent Cancer 

      Kim, Woojin; Bird, Gregory H.; Neff, Tobias; Guo, Guoji; Kerenyi, Marc A.; Walensky, Loren D.; Orkin, Stuart H. (2013)
      Enhancer of zeste homolog2 (EZH2) is the histone lysine N-methyltransferase component of the Polycomb repressive complex 2 (PRC2), which in conjunction with embryonic ectoderm development (EED) and suppressor of zeste 12 ...

    • TiF1-Gamma Plays an Essential Role in Murine Hematopoiesis and Regulates Transcriptional Elongation of Erythroid Genes 

      Bai, Xiaoying; Trowbridge, Jennifer J.; Riley, Elizabeth Leman; Lee, Joseph A.; DiBiase, Anthony; Kaartinen, Vesa M.; Orkin, Stuart Holland; Zon, Leonard Ira (Elsevier BV, 2013)
      Transcriptional regulators play critical roles in the regulation of cell fate during hematopoiesis. Previous studies in zebrafish have identified an essential role for the transcriptional intermediary factor TIF1γ in ...

    • TIF1γ Controls Erythroid Cell Fate by Regulating Transcription Elongation 

      Bai, Xiaoying; Kim, Jonghwan; Yang, Zhongan; Jurynec, Michael J.; Akie, Thomas E.; Lee, Joseph; LeBlanc, Jocelyn; Sessa, Anna; Jiang, Hong; DiBiase, Anthony; Zhou, Yi; Grunwald, David J.; Lin, Shuo; Cantor, Alan B.; Orkin, Stuart Holland; Zon, Leonard Ira (Elsevier BV, 2010)
      Recent genome-wide studies have demonstrated that pausing of RNA polymerase II (Pol II) occurred on many vertebrate genes. By genetic studies in the zebrafish tif1γ mutant moonshine we found that loss of function of Pol ...

    • TMEM14C is required for erythroid mitochondrial heme metabolism 

      Yien, Yvette Yee; Robledo, Raymond F.; Schultz, Iman J.; Takahashi-Makise, Naoko; Gwynn, Babette; Bauer, Daniel Evan; Dass, Abhishek; Yi, Gloria; Li, Liangtao; Hildick-Smith, Gordon J.; Cooney, Jeffrey D.; Pierce, Eric Adam; Mohler, Kyla; Dailey, Tamara A.; Miyata, Non; Kingsley, Paul D.; Garone, Caterina; Hattangadi, Shilpa M.; Huang, Hui; Chen, Wen; Keenan, Ellen M.; Shah, Dhvanit I; Schlaeger, Thorsten M.; DiMauro, Salvatore; Orkin, Stuart Holland; Cantor, Alan B.; Palis, James; Koehler, Carla M.; Lodish, Harvey F.; Kaplan, Jerry B.; Ward, Diane M.; Dailey, Harry A.; Phillips, John Gary; Peters, Luanne L.; Paw, Barry Htin (American Society for Clinical Investigation, 2014)
      The transport and intracellular trafficking of heme biosynthesis intermediates are crucial for hemoglobin production, which is a critical process in developing red cells. Here, we profiled gene expression in terminally ...

    • A Transient Transgenic RNAi Strategy for Rapid Characterization of Gene Function during Embryonic Development 

      Bjork, Bryan Cary; Fujiwara, Yuko; Davis, Shannon W.; Qiu, Haiyan; Saunders, Thomas L.; Sandy, Peter; Orkin, Stuart Holland; Camper, Sally A.; Beier, David Randolph (Public Library of Science, 2010)
      RNA interference (RNAi) is a powerful strategy for studying the phenotypic consequences of reduced gene expression levels in model systems. To develop a method for the rapid characterization of the developmental consequences ...

    • Unraveling the Transcriptional Network Controlling ES Cell Pluripotency 

      Rao, Sridhar; Orkin, Stuart Holland (BioMed Central, 2006)
      Embryonic stem cells (ES cells) are powerful tools for genetic engineering and hold significant potential for regenerative medicine. Recent work provides new insights into ES cell pluripotency and delineates separate ...