Now showing items 21-33 of 33

    • Musashi-2 controls cell fate, lineage bias, and TGF-β signaling in HSCs 

      Park, Sun-Mi; Deering, Raquel P.; Lu, Yuheng; Tivnan, Patrick; Lianoglou, Steve; Al-Shahrour, Fatima; Ebert, Benjamin L.; Hacohen, Nir; Leslie, Christina; Daley, George Q.; Lengner, Christopher J.; Kharas, Michael G. (The Rockefeller University Press, 2014)
      Hematopoietic stem cells (HSCs) are maintained through the regulation of symmetric and asymmetric cell division. We report that conditional ablation of the RNA-binding protein Msi2 results in a failure of HSC maintenance ...
    • Mutant Induced Pluripotent Stem Cell Lines Recapitulate Aspects of TDP-43 Proteinopathies and Reveal Specific Vulnerability 

      Shaw, Christopher E.; Chandran, Siddharthan; Bilican, B.; Serio, A.; Barmada, S. J.; Nishimura, A. L.; Sullivan, G. J.; Carrasco, M.; Phatnani, P.; Friedman, Brad A.; Puddifoot, C. A.; Story, D.; Fletcher, J.; Park, I. H.; Daley, George Quentin; Wyllie, D. J. A.; Hardingham, G. E.; Finkbeiner, S.; Wilmut, I.; Finkbeiner, S.; Maniatis, Thomas P. (National Academy of Sciences, 2012)
      Transactive response DNA-binding (TDP-43) protein is the dominant disease protein in amyotrophic lateral sclerosis (ALS) and a sub-group of frontotemporal lobar degeneration (FTLD-TDP). Identification of TARDBP mutations ...
    • Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway 

      Xu, Cong; Fan, Zi Peng; Müller, Patrick; Fogley, Rachel; DiBiase, Anthony; Trompouki, Eirini; Unternaehrer, Juli; Xiong, Fengzhu; Torregroza, Ingrid; Evans, Todd; Megason, Sean; Daley, George Quentin; Schier, Alexander F; Young, Richard A.; Zon, Leonard Ira (Elsevier, 2012)
      In mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role ...
    • Optimization of scarless human stem cell genome editing 

      Yang, Luhan; Guell, Marc; Byrne, Susan; Yang, Joyce L.; De Los Angeles, Alejandro; Mali, Prashant; Aach, John; Kim-Kiselak, Caroline; Briggs, Adrian W; Rios, Xavier; Huang, Po-Yi; Daley, George; Church, George (Oxford University Press, 2013)
      Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific ...
    • Precise let-7 expression levels balance organ regeneration against tumor suppression 

      Wu, Linwei; Nguyen, Liem H; Zhou, Kejin; de Soysa, T Yvanka; Li, Lin; Miller, Jason B; Tian, Jianmin; Locker, Joseph; Zhang, Shuyuan; Shinoda, Gen; Seligson, Marc T; Zeitels, Lauren R; Acharya, Asha; Wang, Sam C; Mendell, Joshua T; He, Xiaoshun; Nishino, Jinsuke; Morrison, Sean J; Siegwart, Daniel J; Daley, George Q; Shyh-Chang, Ng; Zhu, Hao (eLife Sciences Publications, Ltd, 2015)
      The in vivo roles for even the most intensely studied microRNAs remain poorly defined. Here, analysis of mouse models revealed that let-7, a large and ancient microRNA family, performs tumor suppressive roles at the expense ...
    • Regulation of Stem Cell Metabolism by the Lin28/let-7 Axis 

      Ng, Shyh Chang (2014-02-25)
      My PhD thesis is focused on two fundamental aspects of stem cell metabolism: (1) the role of Lin28 in programming stem cell metabolism, and (2) how metabolism in turn fuels and governs pluripotency. Our studies led us to ...
    • Reprogramming of T Cells From Human Peripheral Blood 

      Loh, Yuin-Han; Hartung, Odelya; Li, Hu; Guo, Chunguang; Sahalie, Julie M.; Manos, Philip D.; Urbach, Achia; Heffner, Garrett C.; Grskovic, Marica; Vigneault, Francois; Lensch, Willy; Park, In-Hyun; Agarwal, Suneet; Church, George; Collins, James; Irion, Stefan; Daley, George (Elsevier BV, 2010-07-02)
    • Reprogramming Pediatric Genetic Disorders: Pearson Syndrome, Ring 14 Syndrome, and Fanconi Anemia 

      Cherry, Anne Blanche Cresswell (2014-06-06)
      The effect of a single genetic mutation can vary greatly between different types of cells. The mutated gene may not be expressed in one tissue but may cause a devastating loss of function in another. To learn about disease ...
    • A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells 

      Lee, Je-Hyuk; Park, In-Hyun; Gao, Yuan; Zhang, Kun; Li, Jin; Li, Zhe; Daley, George Quentin; Church, George McDonald (Public Library of Science, 2009)
      Normal variation in gene expression due to regulatory polymorphisms is often masked by biological and experimental noise. In addition, some regulatory polymorphisms may become apparent only in specific tissues. We derived ...
    • The role of Lin28b in myeloid and mast cell differentiation and mast cell malignancy 

      Wang, Leo; Rao, Tirzah; Rowe, Robert Grant; Nguyen, Phi; Sullivan, Jessica J; Pearson, Daniel Stark; Doulatov, Sergei; Wu, Linwei; Lindsley, Robert Coleman; Zhu, Hao; DeAngelo, Daniel J.; Daley, George Quentin; Wagers, Amy Jo (Nature Publishing Group, 2015)
      Mast cells are critical components of the innate immune system and important for host 48 defense, allergy, autoimmunity, tissue regeneration, and tumor progression.Dysregulated 49 mastcell development leads to systemic ...
    • Targeted and Genome-Scale Methylomics Reveals Gene Body Signatures in Human Cell Lines 

      Ball, Madeleine Price; Li, Jin Billy; Gao, Yuan; Lee, Je-Hyuk; LeProust, Emily; Park, In-Hyun; Xie, Bin; Daley, George Quentin; Church, George McDonald (Nature Publishing Group, 2009)
      Cytosine methylation, an epigenetic modification of DNA, is a target of growing interest for developing high throughput profiling technologies. Here we introduce two new, complementary techniques for cytosine methylation ...
    • Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming 

      Deng, Jie; Shoemaker, Robert; Xie, Bin; Gore, Athurva; LeProust, Emily M; Antosiewicz-Bourget, Jessica; Egli, Dieter; Maherali, Nimet A; Park, In-Hyun; Yu, Junying; Daley, George Quentin; Eggan, Kevin Carl; Hochedlinger, Konrad; Thomson, James; Wang, Wei Li; Gao, Yuan; Zhang, Kun (Nature Publishing Group, 2009)
      Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets. We report a method to specifically capture an arbitrary subset of genomic targets for ...
    • Zcchc11 Uridylates Mature miRNAs to Enhance Neonatal IGF-1 Expression, Growth, and Survival 

      Jones, Matthew R.; Blahna, Matthew T.; Kozlowski, Elyse; Matsuura, Kori Y.; Ferrari, Joseph D.; Morris, Samantha A; Powers, John T; Daley, George Quentin; Quinton, Lee J.; Mizgerd, Joseph P. (Public Library of Science, 2012)
      The Zcchc11 enzyme is implicated in microRNA (miRNA) regulation. It can uridylate let-7 precursors to decrease quantities of the mature miRNA in embryonic stem cell lines, suggested to mediate stem cell maintenance. It can ...