Now showing items 21-29 of 29

    • Multiplex Cytological Profiling Assay to Measure Diverse Cellular States 

      Gustafsdottir, Sigrun M.; Ljosa, Vebjorn; Sokolnicki, Katherine L.; Anthony Wilson, J.; Walpita, Deepika; Kemp, Melissa M.; Petri Seiler, Kathleen; Carrel, Hyman A.; Golub, Todd R.; Schreiber, Stuart L.; Clemons, Paul A.; Carpenter, Anne E.; Shamji, Alykhan F. (Public Library of Science, 2013)
      Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that ...
    • Mutational heterogeneity in cancer and the search for new cancer genes 

      Lawrence, Michael S.; Stojanov, Petar; Polak, Paz; Kryukov, Gregory V.; Cibulskis, Kristian; Sivachenko, Andrey; Carter, Scott L.; Stewart, Chip; Mermel, Craig H.; Roberts, Steven A.; Kiezun, Adam; Hammerman, Peter S.; McKenna, Aaron; Drier, Yotam; Zou, Lihua; Ramos, Alex H.; Pugh, Trevor J.; Stransky, Nicolas; Helman, Elena; Kim, Jaegil; Sougnez, Carrie; Ambrogio, Lauren; Nickerson, Elizabeth; Shefler, Erica; Cortés, Maria L.; Auclair, Daniel; Saksena, Gordon; Voet, Douglas; Noble, Michael; DiCara, Daniel; Lin, Pei; Lichtenstein, Lee; Heiman, David I.; Fennell, Timothy; Imielinski, Marcin; Hernandez, Bryan; Hodis, Eran; Baca, Sylvan; Dulak, Austin M.; Lohr, Jens; Landau, Dan-Avi; Wu, Catherine J.; Melendez-Zajgla, Jorge; Hidalgo-Miranda, Alfredo; Koren, Amnon; McCarroll, Steven A.; Mora, Jaume; Crompton, Brian; Onofrio, Robert; Parkin, Melissa; Winckler, Wendy; Ardlie, Kristin; Gabriel, Stacey B.; Roberts, Charles W. M.; Biegel, Jaclyn A.; Stegmaier, Kimberly; Bass, Adam J.; Garraway, Levi A.; Meyerson, Matthew; Golub, Todd R.; Gordenin, Dmitry A.; Sunyaev, Shamil; Lander, Eric S.; Getz, Gad (2014)
      Major international projects are now underway aimed at creating a comprehensive catalog of all genes responsible for the initiation and progression of cancer. These studies involve sequencing of matched tumor–normal samples ...
    • Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors 

      Chattopadhyay, Shrikanta; Stewart, Alison L.; Mukherjee, Siddhartha; Huang, Cherrie; Hartwell, Kimberly A.; Miller, Peter Grant; Subramanian, Radhika; Carmody, Leigh C.; Yusuf, Rushdia Zareen; Sykes, David Brian; Paulk, Joshiawa; Vetere, Amedeo; Vallet, Sonia; Santo, Loredana; Cirstea, Diana D.; Hideshima, Teru; Dan?ík, Vlado; Majireck, Max M.; Hussain, Mahmud M.; Singh, Shambhavi; Quiroz, Ryan; Iaconelli, Jonathan; Karmacharya, Rakesh; Tolliday, Nicola J.; Clemons, Paul A.; Moore, Malcolm A.S.; Stern, Andrew M.; Shamji, Alykhan Farid; Ebert, Benjamin L.; Golub, Todd Robert; Raje, Noopur; Scadden, David Thomas; Schreiber, Stuart L. (Elsevier BV, 2015)
      Novel therapeutic approaches are urgently required for multiple myeloma (MM). We used a phenotypic screening approach using co-cultures of MM cells with bone marrow stromal cells to identify compounds that overcome stromal ...
    • PAK1 is a Breast Cancer Oncogene that Coordinately Activates MAPK and MET Signaling 

      Shrestha, Yashaswi; Schafer, Eric J.; Boehm, Jesse S.; He, Frank; Wang, Shumei; Barretina, Jordi; Thomas, Sapana Rachael; Du, Jinyan; Weir, Barbara Ann; Zhao, Jean J.; Golub, Todd R.; Beroukhim, Rameen; Hahn, William C.; Polyak, Kornelia (Nature Publishing Group, 2012)
      Activating mutations in the RAS family or BRAF frequently occur in many types of human cancers but are rarely detected in breast tumors. However, activation of the RAS-RAF-MEK-ERK Mitogen-Activated Protein Kinase (MAPK) ...
    • Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies 

      Cowley, Glenn S; Weir, Barbara A; Vazquez, Francisca; Tamayo, Pablo; Scott, Justine A; Rusin, Scott; East-Seletsky, Alexandra; Ali, Levi D; Gerath, William FJ; Pantel, Sarah E; Lizotte, Patrick H; Jiang, Guozhi; Hsiao, Jessica; Tsherniak, Aviad; Dwinell, Elizabeth; Aoyama, Simon; Okamoto, Michael; Harrington, William; Gelfand, Ellen; Green, Thomas M; Tomko, Mark J; Gopal, Shuba; Wong, Terence C; Li, Hubo; Howell, Sara; Stransky, Nicolas; Liefeld, Ted; Jang, Dongkeun; Bistline, Jonathan; Hill Meyers, Barbara; Armstrong, Scott A; Anderson, Ken C; Stegmaier, Kimberly; Reich, Michael; Pellman, David; Boehm, Jesse S; Mesirov, Jill P; Golub, Todd R; Root, David E; Hahn, William C (Nature Publishing Group, 2014)
      Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell ...
    • Signature-Based Small Molecule Screening Identifies Cytosine Arabinoside as an EWS/FLI Modulator in Ewing Sarcoma 

      Stegmaier, Kimberly; Wong, Jenny S; Ross, Kenneth N; Chow, Kwan T; Peck, David; Wright, Renee D; Lessnick, Stephen L; Kung, Andrew Li-Jen; Golub, Todd R. (Public Library of Science, 2007)
      Background: The presence of tumor-specific mutations in the cancer genome represents a potential opportunity for pharmacologic intervention to therapeutic benefit. Unfortunately, many classes of oncoproteins (e.g., ...
    • Tumor microenvironment induces innate RAF-inhibitor resistance through HGF secretion 

      Straussman, Ravid; Morikawa, Teppei; Shee, Kevin; Barzily-Rokni, Michal; Qian, Zhi Rong; Du, Jinyan; Davis, Ashli; Mongare, Margaret M.; Gould, Joshua; Frederick, Dennie T.; Cooper, Zachary A.; Chapman, Paul B.; Solit, David B.; Ribas, Antoni; Lo, Roger S.; Flaherty, Keith T.; Ogino, Shuji; Wargo, Jennifer A.; Golub, Todd R. (2012)
      Drug resistance remains a vexing problem in the treatment of cancer patients. While many studies have focused on cell autonomous mechanisms of drug resistance, we hypothesized that the tumor microenvironment may confer ...
    • Whole exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer 

      Lohr, Jens G.; Adalsteinsson, Viktor A.; Cibulskis, Kristian; Choudhury, Atish D.; Rosenberg, Mara; Cruz-Gordillo, Peter; Francis, Joshua; Zhang, Cheng-Zhong; Shalek, Alex K.; Satija, Rahul; Trombetta, John T.; Lu, Diana; Tallapragada, Naren; Tahirova, Narmin; Kim, Sora; Blumenstiel, Brendan; Sougnez, Carrie; Lowe, Alarice; Wong, Bang; Auclair, Daniel; Van Allen, Eliezer M.; Nakabayashi, Mari; Lis, Rosina T.; Lee, Gwo-Shu M.; Li, Tiantian; Chabot, Matthew S.; Ly, Amy; Taplin, Mary-Ellen; Clancy, Thomas E.; Loda, Massimo; Regev, Aviv; Meyerson, Matthew; Hahn, William C.; Kantoff, Philip W.; Golub, Todd R.; Getz, Gad; Boehm, Jesse S.; Love, J. Christopher (2014)
      Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. A major barrier, however, is inaccessibility of metastatic tissue. A potential solution is to characterize circulating ...
    • WRN Helicase is a Synthetic Lethal Target in Microsatellite Unstable Cancers 

      Bass, Adam; Chan, Edmond; Shibue, Tsukasa; McFarland, James; Gaeta, Benjamin; Ghandi, Mahmoud; Dumont, Nancy; Gonzalez, Alfredo; McPartlan, Justine; Li, Tianxia; Zhang, Yanxi; Liu, Jie Bin; Lazaro, Jean-Bernard; Gu, Peili; Piett, Cortt; Apffel, Annie; Ali, Syed Omar; Deasy, Rebecca; Keskula, Paula; Ng, Raymond; Roberts, Emma; Reznichenko, Elizaveta; Leung, Lisa; Alimova, Maria; Schenone, Monica; Islam, Manirul; Maruvka, Yosef; Liu, Yang; Roper, Jatin; Raghavan, Srivatsan; Giannakis, Marios; Tseng, Yuen-Yi; Nagel, Zachary; D’Andrea, Alan; Root, David; Boehm, Jesse; Getz, Gad; Chang, Sandy; Golub, Todd; Tsherniak, Aviad; Vazquez, Francisca (Cold Spring Harbor Laboratory, 2018-12-21)
      Synthetic lethality, an interaction whereby the co-occurrence of two genetic events leads to cell death but one event alone does not, can be exploited for cancer therapeutics1. DNA repair processes represent attractive ...