Now showing items 1-20 of 184

    • Abnormal Hair Development and Apparent Follicular Transformation to Mammary Gland in the Absence of Hedgehog Signaling 

      Gritli-Linde, Amel; Linde, Anders; Hallberg, Kristina; Harfe, Brian D.; Reyahi, Azadeh; Kannius-Janson, Marie; Nilsson, Jeanette; Cobourne, Martyn T.; Sharpe, Paul T.; McMahon, Andrew P. (Elsevier, 2007)
      Summary: We show that removing the Shh signal tranducer Smoothened from skin epithelium secondarily results in excess Shh levels in the mesenchyme. Moreover, the phenotypes we observe reflect decreased epithelial Shh ...
    • Adenosine kinase inhibition selectively promotes rodent and porcine islet β-cell replication 

      Annes, J. P.; Ryu, J. H.; Lam, K.; Carolan, Peter Joseph; Utz, K.; Hollister-Lock, J.; Anthony, Anthony; Rubin, Lee; Weir, Gordon Campbell; Melton, Douglas A. (Proceedings of the National Academy of Sciences, 2012)
      Diabetes is a pathological condition characterized by relative insulin deficiency, persistent hyperglycemia, and, consequently, diffuse micro- and macrovascular disease. One therapeutic strategy is to amplify insulin-secretion ...
    • Alzheimer's disease: early alterations in brain DNA methylation at ANK1, BIN1, RHBDF2 and other loci 

      De Jager, Philip Lawrence; Srivastava, Gyan; Lunnon, Katie; Burgess, Jeremy; Schalkwyk, Leonard C; Yu, Lei; Eaton, Matthew L; Keenan, Brendan T; Ernst, Jason; McCabe, Cristin; Tang, Anna; Raj, Towfique; Replogle, Joseph; Brodeur, Wendy; Gabriel, Stacey; Chai, High S; Younkin, Curtis; Younkin, Steven G; Zou, Fanggeng; Szyf, Moshe; Epstein, Charles B; Schneider, Julie A; Bernstein, Bradley E.; Meissner, Alexander; Ertekin-Taner, Nilufer; Chibnik, Lori; Kellis, Manolis; Mill, Jonathan; Bennett, David A (Nature Publishing Group, 2014)
      We used a collection of 708 prospectively collected autopsied brains to assess the methylation state of the brain's DNA in relation to Alzheimer's disease (AD). We found that the level of methylation at 71 of the 415,848 ...
    • Anatomic and Molecular Development of Corticostriatal Projection Neurons in Mice 

      Sohur, Usharbudh Shivraj; Padmanabhan, Hari; Kotchetkov, Ivan S.; Menezes, Joao R.L.; Macklis, Jeffrey Daniel (Oxford University Press, 2013-03-18)
      Corticostriatal projection neurons (CStrPN) project from the neocortex to ipsilateral and contralateral striata to control and coordinate motor programs and movement. They are clinically important as the predominant cortical ...
    • Area-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI 

      Tomassy, G. S.; De Leonibus, E.; Jabaudon, D.; Lodato, Simona; Alfano, C.; Mele, A.; Macklis, Jeffrey Daniel; Studer, M. (Proceedings of the National Academy of Sciences, 2010)
      Transcription factors with gradients of expression in neocortical progenitors give rise to distinct motor and sensory cortical areas by controlling the area-specific differentiation of distinct neuronal subtypes. However, ...
    • Bayesian approach to single-cell differential expression analysis 

      Kharchenko, Peter Vasili; Silberstein, Lev; Scadden, David Thomas (Springer Nature, 2014)
      Single-cell data provide a means to dissect the composition of complex tissues and specialized cellular environments. However, the analysis of such measurements is complicated by high levels of technical noise and intrinsic ...
    • BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells 

      Schemionek, M.; Elling, C.; Steidl, U.; Baumer, N.; Hamilton, A.; Spieker, T.; Gothert, J. R.; Stehling, M.; Wagers, Amy Jo; Huettner, Claudia Sonja; Tenen, Daniel Geoffrey; Tickenbrock, L.; Berdel, W. E.; Serve, H.; Holyoake, T. L.; Muller-Tidow, C.; Koschmieder, S. (American Society of Hematology, 2010)
      In a previously developed inducible transgenic mouse model of chronic myeloid leukemia, we now demonstrate that the disease is transplantable using BCR-ABL+ Lin−Sca-1+c-kit+ (LSK) cells. Interestingly, the phenotype is ...
    • Betatrophin: A Hormone that Controls Pancreatic β Cell Proliferation 

      Yi, Peng; Park, Ji Sun; Melton, Douglas A. (Elsevier BV, 2013)
      Replenishing insulin-producing pancreatic β cell mass will benefit both type I and type II diabetics. In adults, pancreatic β cells are generated primarily by self-duplication. We report on a mouse model of insulin resistance ...
    • Bhlhb5 Regulates the Postmitotic Acquisition of Area Identities in Layers II-V of the Developing Neocortex 

      Joshi, Pushkar S.; Molyneaux, Bradley John; Feng, Liang; Xie, Xiaoliang Sunney; Macklis, Jeffrey Daniel; Gan, Lin (Elsevier, 2008)
      While progenitor-restricted factors broadly specify area identities in developing neocortex, the downstream regulatory elements involved in acquisition of those identities in post-mitotic neurons are largely unknown. Here, ...
    • Braveheart, a Long Noncoding RNA Required for Cardiovascular Lineage Commitment 

      Klattenhoff, Carla A.; Scheuermann, Johanna C.; Surface, Lauren E.; Bradley, Robert K.; Fields, Paul A.; Steinhauser, Matthew L.; Ding, Huiming; Butty, Vincent L.; Torrey, Lillian; Haas, Simon; Abo, Ryan; Tabebordbar, Mohammadsharif; Lee, Richard Theodore; Burge, Christopher B.; Boyer, Laurie A. (Elsevier BV, 2013)
      Long noncoding RNAs (lncRNAs) are often expressed in a development-specific manner, yet little is known about their roles in lineage commitment. Here, we identified Braveheart (Bvht), a heart-associated lncRNA in mouse. ...
    • Brief Report: VGLL4 Is a Novel Regulator of Survival in Human Embryonic Stem Cells 

      Tajonar, Adriana; Maehr, René; Hu, Guang; Sneddon, Julie Beth; Rivera-Feliciano, Jose; Cohen, Dena Elana; Elledge, Stephen J.; Melton, Douglas A. (AlphaMed Press, 2013)
      Human embryonic stem cells (hESCs) are maintained in a self-renewing state by an interconnected network of mechanisms that sustain pluripotency, promote proliferation and survival, and prevent differentiation. We sought ...
    • Cardiac Stem Cell Therapy and the Promise of Heart Regeneration 

      Garbern, Jessica; Lee, Richard Theodore (Elsevier BV, 2013)
      Stem cell therapy for cardiac disease is an exciting but highly controversial research area. Strategies such as cell transplantation and reprogramming have demonstrated both intriguing and sobering results. Yet as clinical ...
    • Cbx Proteins Help ESCs Walk the Line between Self-Renewal and Differentiation 

      Camahort, Raymond; Cowan, Chad A. (Elsevier BV, 2012)
      The Polycomb repressive complexes (PRC) regulate self-renewal and differentiation in embryonic stem cells (ESCs). In this issue of "Cell Stem Cell," Morey et al. (2012) and O'Loghlen et al. (2012) report that dynamic ...
    • The cdx Genes and Retinoic Acid Control the Positioning and Segmentation of the Zebrafish Pronephros 

      Selleck, Rori; Song, Huai-Dong; Song, Anhua; Thisse, Bernard; Thisse, Christine; Mullins, Mary; Wingert, Rebecca Ann; Yu, Jingyi; Chen, Zhu; Zhou, Yi; McMahon, Andrew P.; Davidson, Alan J. (Public Library of Science, 2007)
      Kidney function depends on the nephron, which comprises a blood filter, a tubule that is subdivided into functionally distinct segments, and a collecting duct. How these regions arise during development is poorly understood. ...
    • Cdx4 Is Dispensable for Murine Adult Hematopoietic Stem Cells but Promotes MLL-AF9-Mediated Leukemogenesis 

      Koo, Sumin; Huntly, Brian J.; Wang, Yuan; Chen, Jing; Brumme, Kristina; Ball, Brian; McKinney-Freeman, Shannon L.; Yabuuchi, Akiko; Scholl, Claudia; Bansal, Dimple; Zon, Leonard Ira; Fröhling, Stefan; Daley, George Quentin; Gilliland, D. Gary; Mercher, Thomas (Ferrata Storti Foundation (Haematologica), 2010)
      Background: Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior Hox genes. However, its role in adult mammalian hematopoiesis has not ...
    • Cell Type of Origin Influences the Molecular and Functional Properties of Mouse Induced Pluripotent Stem Cells 

      Polo, Jose M.; Liu, Susanna; Figueroa, Maria Eugenia; Kulalert, Warakorn; Eminli, Sarah; Tan, Kah Yong; Apostolou, Effie; Stadtfeld, Matthias; Li, Yushan; Shioda, Toshihiro; Natesan, Sridaran; Wagers, Amy Jo; Melnick, Ari; Evans, Todd; Hochedlinger, Konrad (Nature Publishing Group, 2010)
      Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly ...
    • Characterization of immune-matched hematopoietic transplantation in zebrafish 

      de Jong, J. L. O.; Burns, Caroline Erter; Chen, A. T.; Pugach, E.; Mayhall, E. A.; Smith, A. C. H.; Feldman, Henry Arthur; Zhou, Yi; Zon, Leonard Ira (American Society of Hematology, 2011)
      Evaluating hematopoietic stem cell (HSC) function in vivo requires a long-term transplantation assay. Although zebrafish are a powerful model for discovering the genetics of hematopoiesis, hematopoietic transplantation ...
    • A Chemical Genetic Screen in Zebrafish for Pathways Interacting with cdx4 in Primitive Hematopoiesis 

      Paik, Elizabeth Jae-Eun; de Jong, Jill L.O.; Pugach, Emily; Opara, Praise; Zon, Leonard Ira (Mary Ann Liebert Inc, 2010)
      cdx4, a caudal-related homeodomain-containing transcription factor, functions as a regulator of hox genes, thereby playing a critical role in anterior–posterior (A-P) patterning during embryogenesis. In zebrafish, homozygous ...
    • Circulating C3 is Necessary and Sufficient for Induction of Autoantibody-Mediated Arthritis in a Mouse Model 

      Monach, Paul A; Verschoor, Admar; Jacobs, Jonathan P; Carroll, Michael C.; Wagers, Amy Jo; Benoist, Christophe O.; Mathis, Diane J. (John Wiley & Sons, 2007)
      Objective. For the inflammation characteristic of rheumatoid arthritis, the relative contribution of mediators produced locally in the synovium versus those circulating systemically is unknown. Complement factor C3 is made ...
    • Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity 

      Fame, Ryan Marie; MacDonald, Jessica Linn; Dunwoodie, S. L.; Oki, Emi Takahashi; Macklis, Jeffrey Daniel (Society for Neuroscience, 2016)
      The neocortex contains hundreds to thousands of distinct subtypes of precisely connected neurons, allowing it to perform remarkably complex tasks of high-level cognition. Callosal projection neurons (CPN) connect the ...