Now showing items 1-12 of 12

• #### Characterization of Systemic RNAi Mutants in Caenorhabditis Elegans ﻿

(2019-05-17)
RNA interference (RNAi) is sequence-specific gene silencing triggered by double-stranded (ds)RNA. When dsRNA is expressed or introduced into one cell and is transported to and initiates RNAi in other cells, it is called ...
• #### Characterizing chemosensory responses in C. elegans with multi-neuronal imaging ﻿

(2021-07-12)
How do animals use their sensory neurons to perceive their chemical environment? How does sensory information get transformed by downstream circuits? And ultimately, how do animals generate behavior in response to chemical ...
• #### Dietary Restriction-Induced Longevity in Caenorhabditis elegans: Mediated by Stress Defense, NAD$^+$-Dependent Mechanisms and a Respiratory Shift ﻿

(2014-06-06)
Dietary restriction (DR), or the reduction of food consumption without malnutrition, is the most conserved method of preventing or reversing age associated diseases. It is also the most conserved method of increasing ...
• #### Ether Lipid Biosynthesis Promotes Lifespan Extension and Enables Diverse Prolongevity Paradigms ﻿

(2022-05-10)
Biguanides, including the world’s most commonly prescribed drug for type 2 diabetes, metformin, not only lower blood sugar, but also promote longevity in preclinical models. Epidemiologic studies in humans parallel these ...
• #### Modulation of small RNA silencing by cross-generational signaling in C. elegans ﻿

(2014-06-06)
Organisms are constantly challenged by the surrounding environment and alter their physiology accordingly. Some environment-induced changes in one generation are inherited in the offspring, and this long-lasting memory of ...
• #### Molecular and Circuit Mechanisms of Insulin Signaling in Caenorhabditis elegans ﻿

(2014-02-25)
Insulin signaling is highly conserved across animals, and is known for its ubiquitous function in all aspects of animal physiology. Despite its relatively well-studied role in metabolism and energy expenditure, how it is ...
• #### Optogenetics and Computer Vision for C. elegans Neuroscience and Other Biophysical Applications ﻿

(2012-07-19)
This work presents optogenetics and real-time computer vision techniques to non-invasively manipulate and monitor neural activity with high spatiotemporal resolution in awake behaving Caenorhabditis elegans. These methods ...
• #### Pairing of Competitive and Topologically Distinct Regulatory Modules Enhances Patterned Gene Expression ﻿

(Nature Publishing Group, 2008)
Biological networks are inherently modular, yet little is known about how modules are assembled to enable coordinated and complex functions. We used RNAi and time series, whole-genome microarray analyses to systematically ...
• #### Pan-neuronal imaging in roaming Caenorhabditis elegans ﻿

(Proceedings of the National Academy of Sciences, 2015)
We present an imaging system for pan-neuronal recording in crawling Caenorhabditis elegans. A spinning disk confocal microscope, modified for automated tracking of the C. elegans head ganglia, simultaneously records the ...
• #### Spatio-temporal control of the cytosolic redox environment in C. elegans ﻿

(2013-10-18)
Compartmentalization of redox reactions is essential to all life forms. Protein activity can respond to changes in the local redox environment through the reversible oxidation of cysteine thiols. For the majority of cysteines ...
• #### The role of RNA splicing factors in the regulation of longevity ﻿

(2021-11-16)
Geroscience aims to target the aging process to extend healthspan. However, the efficacy of pro- longevity interventions is highly heterogeneous, limiting their translational potential. In my dissertation research, I ...
• #### Transcriptional Regulation of Synapse Remodeling in C. elegans ﻿

(2012-12-19)
The ability of a neuron to alter its synaptic connections during development is essential to circuit assembly. Synapse remodeling or refinement has been observed in many species and many neuronal circuits, yet the mechanisms ...