Now showing items 1-20 of 31

    • Bioelectric Signaling Regulates Size in Zebrafish Fins 

      Perathoner, Simon; Daane, Jacob M.; Henrion, Ulrike; Seebohm, Guiscard; Higdon, Charles W.; Johnson, Stephen L.; Nüsslein-Volhard, Christiane; Harris, Matthew P. (Public Library of Science, 2014)
      The scaling relationship between the size of an appendage or organ and that of the body as a whole is tightly regulated during animal development. If a structure grows at a different rate than the rest of the body, this ...
    • Brain-wide mapping of neural activity controlling zebrafish exploratory locomotion 

      Dunn, Timothy W; Mu, Yu; Narayan, Sujatha; Randlett, Owen; Naumann, Eva A; Yang, Chao-Tsung; Schier, Alexander F; Freeman, Jeremy; Engert, Florian; Ahrens, Misha B (eLife Sciences Publications, Ltd, 2016)
      In the absence of salient sensory cues to guide behavior, animals must still execute sequences of motor actions in order to forage and explore. How such successive motor actions are coordinated to form global locomotion ...
    • Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons 

      Sheets, Lavinia; Hagen, Matthew W.; Nicolson, Teresa (Public Library of Science, 2014)
      Synaptic ribbons are presynaptic structures formed by the self-association of RIBEYE–the main structural component of ribbon synapses. RIBEYE consists of two domains: a unique N-terminal A-domain and a C-terminal B-domain ...
    • A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development 

      Hill, Jennifer Hampton; Franzosa, Eric A; Huttenhower, Curtis; Guillemin, Karen (eLife Sciences Publications, Ltd, 2016)
      Resident microbes play important roles in the development of the gastrointestinal tract, but their influence on other digestive organs is less well explored. Using the gnotobiotic zebrafish, we discovered that the normal ...
    • Dimethyl Sulfoxide (DMSO) Exacerbates Cisplatin-induced Sensory Hair Cell Death in Zebrafish (Danio rerio) 

      Uribe, Phillip M.; Mueller, Melissa; Gleichman, Julia S.; Kramer, Matthew; Wang, Qi; Sibrian-Vazquez, Martha; Strongin, Robert M.; Steyger, Peter S.; Cotanche, Douglas; Matsui, Jonathan I. (Public Library of Science, 2013)
      Inner ear sensory hair cells die following exposure to aminoglycoside antibiotics or chemotherapeutics like cisplatin, leading to permanent auditory and/or balance deficits in humans. Zebrafish (Danio rerio) are used to ...
    • Discovery and validation of sub-threshold genome-wide association study loci using epigenomic signatures 

      Wang, Xinchen; Tucker, Nathan R; Rizki, Gizem; Mills, Robert; Krijger, Peter HL; de Wit, Elzo; Subramanian, Vidya; Bartell, Eric; Nguyen, Xinh-Xinh; Ye, Jiangchuan; Leyton-Mange, Jordan; Dolmatova, Elena V; van der Harst, Pim; de Laat, Wouter; Ellinor, Patrick T; Newton-Cheh, Christopher; Milan, David J; Kellis, Manolis; Boyer, Laurie A (eLife Sciences Publications, Ltd, 2016)
      Genetic variants identified by genome-wide association studies explain only a modest proportion of heritability, suggesting that meaningful associations lie 'hidden' below current thresholds. Here, we integrate information ...
    • DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish 

      Liu, Xiaohui; Jia, Xiaoe; Yuan, Hao; Ma, Ke; Chen, Yi; Jin, Yi; Deng, Min; Pan, Weijun; Chen, Saijuan; Chen, Zhu; de The, Hugues; Zon, Leonard I; Zhou, Yi; Zhou, Jun; Zhu, Jun (BioMed Central, 2015)
      Background: DNA methyltransferase 1 (Dnmt1) regulates expression of many critical genes through maintaining parental DNA methylation patterns on daughter DNA strands during mitosis. It is essential for embryonic development ...
    • eduSPIM: Light Sheet Microscopy in the Museum 

      Jahr, Wiebke; Schmid, Benjamin; Weber, Michael; Huisken, Jan (Public Library of Science, 2016)
      Light Sheet Microscopy in the Museum Light sheet microscopy (or selective plane illumination microscopy) is an important imaging technique in the life sciences. At the same time, this technique is also ideally suited for ...
    • Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs 

      Gagnon, James A.; Valen, Eivind; Thyme, Summer B.; Huang, Peng; Ahkmetova, Laila; Pauli, Andrea; Montague, Tessa G.; Zimmerman, Steven; Richter, Constance; Schier, Alexander F. (Public Library of Science, 2014)
      The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at a limited number of genomic target sites. To uncover ...
    • FishFace: interactive atlas of zebrafish craniofacial development at cellular resolution 

      Eames, B Frank; DeLaurier, April; Ullmann, Bonnie; Huycke, Tyler R; Nichols, James T; Dowd, John; McFadden, Marcie; Sasaki, Mark M; Kimmel, Charles B (BioMed Central, 2013)
      Background: The vertebrate craniofacial skeleton may exhibit anatomical complexity and diversity, but its genesis and evolution can be understood through careful dissection of developmental programs at cellular resolution. ...
    • Heart on a Plate: Histological and Functional Assessment of Isolated Adult Zebrafish Hearts Maintained in Culture 

      Pieperhoff, Sebastian; Wilson, Kathryn S.; Baily, James; de Mora, Kim; Maqsood, Sana; Vass, Sharron; Taylor, Jonathan; Del-Pozo, Jorge; MacRae, Calum A.; Mullins, John J.; Denvir, Martin A. (Public Library of Science, 2014)
      The zebrafish is increasingly used for cardiovascular genetic and functional studies. We present a novel protocol to maintain and monitor whole isolated beating adult zebrafish hearts in culture for long-term experiments. ...
    • Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System 

      Hwang, Woong Y.; Fu, Yanfang; Reyon, Deepak; Maeder, Morgan L.; Kaini, Prakriti; Sander, Jeffry D.; Joung, J. Keith; Peterson, Randall T.; Yeh, Jing-Ruey Joanna (Public Library of Science, 2013)
      We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic ...
    • The Histone Methyltransferase SUV39H1 Suppresses Embryonal Rhabdomyosarcoma Formation in Zebrafish 

      Albacker, Colleen Elizabeth; Storer, Narie Yoo; Langdon, Erin M.; DiBiase, Anthony; Zhou, Yi; Langenau, David M.; Zon, Leonard Ira (Public Library of Science, 2013)
      Epigenetics, or the reversible and heritable marks of gene regulation not including DNA sequence, encompasses chromatin modifications on both the DNA and histones and is as important as the DNA sequence itself. Chromatin-modifying ...
    • Histopathologic Alterations Associated with Global Gene Expression Due to Chronic Dietary TCDD Exposure in Juvenile Zebrafish 

      Liu, Qing; Spitsbergen, Jan M.; Cariou, Ronan; Huang, Chun-Yuan; Jiang, Nan; Goetz, Giles; Hutz, Reinhold J.; Tonellato, Peter J.; Carvan, Michael J. (Public Library of Science, 2014)
      The goal of this project was to investigate the effects and possible developmental disease implication of chronic dietary TCDD exposure on global gene expression anchored to histopathologic analysis in juvenile zebrafish ...
    • Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs) 

      Moore, Finola Elizabeth; Reyon, Deepak; Sander, Jeffry D.; Martinez, Sarah A.; Blackburn, Jessica S.; Khayter, Cyd; Ramirez, Cherie Lynn; Joung, Jae Keith; Langenau, David M. (Public Library of Science, 2012)
      Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in ...
    • Interdependence of Bad and Puma during Ionizing-Radiation-Induced Apoptosis 

      Toruno, Cristhian; Carbonneau, Seth; Stewart, Rodney A.; Jette, Cicely (Public Library of Science, 2014)
      Ionizing radiation (IR)-induced DNA double-strand breaks trigger an extensive cellular signaling response that involves the coordination of hundreds of proteins to regulate DNA repair, cell cycle arrest and apoptotic ...
    • Mechanical Stretch and PI3K Signaling Link Cell Migration and Proliferation to Coordinate Epithelial Tubule Morphogenesis in the Zebrafish Pronephros 

      Vasilyev, Aleksandr; Liu, Yan; Hellman, Nathan; Pathak, Narendra H.; Drummond, Iain A. (Public Library of Science, 2012)
      Organ development leads to the emergence of organ function, which in turn can impact developmental processes. Here we show that fluid flow-induced collective epithelial migration during kidney nephron morphogenesis induces ...
    • Microarray Noninvasive Neuronal Seizure Recordings from Intact Larval Zebrafish 

      Meyer, Michaela; Dhamne, Sameer C.; LaCoursiere, Christopher M.; Tambunan, Dimira; Poduri, Annapurna; Rotenberg, Alexander (Public Library of Science, 2016)
      Zebrafish epilepsy models are emerging tools in experimental epilepsy. Zebrafish larvae, in particular, are advantageous because they can be easily genetically altered and used for developmental and drug studies since ...
    • Myc and Fgf Are Required for Zebrafish Neuromast Hair Cell Regeneration 

      Lee, Sang Goo; Huang, Mingqian; Obholzer, Nikolaus D.; Sun, Shan; Li, Wenyan; Petrillo, Marco; Dai, Pu; Zhou, Yi; Cotanche, Douglas A.; Megason, Sean G.; Li, Huawei; Chen, Zheng-Yi (Public Library of Science, 2016)
      Unlike mammals, the non-mammalian vertebrate inner ear can regenerate the sensory cells, hair cells, either spontaneously or through induction after hair cell loss, leading to hearing recovery. The mechanisms underlying ...
    • Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma 

      Tenente, Inês M; Hayes, Madeline N; Ignatius, Myron S; McCarthy, Karin; Yohe, Marielle; Sindiri, Sivasish; Gryder, Berkley; Oliveira, Mariana L; Ramakrishnan, Ashwin; Tang, Qin; Chen, Eleanor Y; Petur Nielsen, G; Khan, Javed; Langenau, David M (eLife Sciences Publications, Ltd, 2017)
      Rhabdomyosarcoma (RMS) is a pediatric malignacy of muscle with myogenic regulatory transcription factors MYOD and MYF5 being expressed in this disease. Consensus in the field has been that expression of these factors likely ...