Now showing items 1-20 of 48

    • Advances in complex multiparameter flow cytometry technology: Applications in stem cell research 

      Preffer, Frederic Ira; Dombkowski, David (Wiley-Blackwell, 2009)
      Flow cytometry and cell sorting are critical tools in stem cell research. Recent advances in flow cytometric hardware, reagents, and software have synergized to permit the stem cell biologist to more full identify and ...
    • Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper 

      Lener, Thomas; Gimona, Mario; Aigner, Ludwig; Börger, Verena; Buzas, Edit; Camussi, Giovanni; Chaput, Nathalie; Chatterjee, Devasis; Court, Felipe A.; del Portillo, Hernando A.; O'Driscoll, Lorraine; Fais, Stefano; Falcon-Perez, Juan M.; Felderhoff-Mueser, Ursula; Fraile, Lorenzo; Gho, Yong Song; Görgens, André; Gupta, Ramesh C.; Hendrix, An; Hermann, Dirk M.; Hill, Andrew F.; Hochberg, Fred; Horn, Peter A.; de Kleijn, Dominique; Kordelas, Lambros; Kramer, Boris W.; Krämer-Albers, Eva-Maria; Laner-Plamberger, Sandra; Laitinen, Saara; Leonardi, Tommaso; Lorenowicz, Magdalena J.; Lim, Sai Kiang; Lötvall, Jan; Maguire, Casey A.; Marcilla, Antonio; Nazarenko, Irina; Ochiya, Takahiro; Patel, Tushar; Pedersen, Shona; Pocsfalvi, Gabriella; Pluchino, Stefano; Quesenberry, Peter; Reischl, Ilona G.; Rivera, Francisco J.; Sanzenbacher, Ralf; Schallmoser, Katharina; Slaper-Cortenbach, Ineke; Strunk, Dirk; Tonn, Torsten; Vader, Pieter; van Balkom, Bas W. M.; Wauben, Marca; Andaloussi, Samir El; Théry, Clotilde; Rohde, Eva; Giebel, Bernd (Co-Action Publishing, 2015)
      Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types and participate in physiological and pathophysiological processes. EVs mediate intercellular communication as cell-derived ...
    • B-MYB Is Essential for Normal Cell Cycle Progression and Chromosomal Stability of Embryonic Stem Cells 

      Tarasov, Kirill V.; Tarasova, Yelena S.; Tam, Wai Leong; Riordon, Daniel R.; Elliott, Steven T.; Kania, Gabriela; Li, Jinliang; Yamanaka, Satoshi; Crider, David G.; Testa, Gianluca; Li, Ronald A.; Stewart, Colin L.; Liu, Yie; Van Eyk, Jennifer E.; Wersto, Robert P.; Wobus, Anna M.; Boheler, Kenneth R.; Lim, Bing (Public Library of Science, 2008)
      Background: The transcription factor B-Myb is present in all proliferating cells, and in mice engineered to remove this gene, embryos die in utero just after implantation due to inner cell mass defects. This lethal phenotype ...
    • The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing 

      Huang, Yun; Pastor, William Abraham; Shen, Yinghua; Tahiliani, Mamta V.; Liu, David Ruchien; Rao, Anjana; Liu, Jun (Public Library of Science, 2010)
      Background: We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons. The methylation status of ...
    • Cancer Stem Cell-Like Cells Derived from Malignant Peripheral Nerve Sheath Tumors 

      Spyra, Melanie; Kluwe, Lan; Hagel, Christian; Nguyen, Rosalyn T.; Panse, Jens; Kurtz, Andreas; Mautner, Victor Felix; Rabkin, Samuel David; Demestre, Maria (Public Library of Science, 2011)
      This study aims to examine whether or not cancer stem cells exist in malignant peripheral nerve sheath tumors (MPNST). Cells of established lines, primary cultures and freshly dissected tumors were cultured in serum free ...
    • Charting and Navigating Fate Decisions in Directed Differentiation of Stem Cell-Derived Human Beta Cells 

      Veres, Adrian (2019-05-17)
      Beta cells are front-runners in the fields of regenerative medicine and directed differentiation. Significant progress has been made in producing beta cells in vitro from pluripotent human cells and using these cells in ...
    • Chromatin Modifying Enzymes as Modulators of Reprogramming 

      Onder, Tamer T.; Kara, Nergis; Sinha, Amit U.; Bernt, Kathrin M.; Mancarci, Ogan. B.; Gupta, Piyush B.; Cherry, Anne Blanche Cresswell; Zhu, Nan; Cahan, Patrick; Unternaehrer, Juli; Lander, Eric Steven; Armstrong, Scott A.; Daley, George Quentin (Nature Publishing Group, 2012)
      Generation of induced pluripotent stem cells (iPSCs) by somatic cell reprogramming involves global epigenetic remodeling. While several proteins are known to regulate chromatin marks associated with the distinct epigenetic ...
    • CIP2A Promotes Proliferation of Spermatogonial Progenitor Cells and Spermatogenesis in Mice 

      Ventelä, Sami; Côme, Christophe; Mäkelä, Juho-Antti; Hobbs, Robin M.; Mannermaa, Leni; Kallajoki, Markku; Chan, Edward K.; Pandolfi, Pier Paolo; Toppari, Jorma; Westermarck, Jukka (Public Library of Science, 2012)
      Protein phosphatase 2A (PP2A) is a critical regulator of protein serine/threonine phosphorylation. However, the physiological and developmental roles of different PP2A complexes are very poorly understood. Here, we show ...
    • Circulating Endothelial Progenitor Cells Are Up-Regulated in a Mouse Model of Endometriosis 

      Becker, Christian M.; Beaudry, Paul; Funakoshi, Tae; Benny, Ofra; Zaslavsky, Alexander; Zurakowski, David; Folkman, Judah; D\'Amato, Robert J.; Ryeom, Sandra (American Society for Investigative Pathology, 2011)
      Endometriosis is a debilitating disease characterized by the growth of ectopic endometrial tissue. It is widely accepted that angiogenesis plays an integral part in the establishment and growth of endometriotic lesions. ...
    • Comparative Lesion Sequencing Provides Insights into Tumor Evolution 

      Jones, Siân; Chen, Wei-dong; Parmigiani, Giovanni; Diehl, Frank; Beerenwinkel, Niko; Antal, Tibor; Traulsen, Arne; Nowak, Martin A.; Siegel, Christopher; Velculescu, Victor E.; Kinzler, Kenneth W.; Vogelstein, Bert; Willis, Joseph; Markowitz, Sanford D. (Proceedings of the National Academy of Sciences, 2008)
      We show that the times separating the birth of benign, invasive, and metastatic tumor cells can be determined by analysis of the mutations they have in common. When combined with prior clinical observations, these analyses ...
    • A Compendium of Preparation and Application of Stem Cells in Parkinson's Disease: Current Status and Future Prospects 

      Shen, Yan; Huang, Jinsha; Liu, Ling; Xu, Xiaoyun; Han, Chao; Zhang, Guoxin; Jiang, Haiyang; Li, Jie; Lin, Zhicheng; Xiong, Nian; Wang, Tao (Frontiers Media S.A., 2016)
      Parkinson's Disease (PD) is a progressively neurodegenerative disorder, implicitly characterized by a stepwise loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc) and explicitly marked by ...
    • Corticotropin-releasing hormone exerts direct effects on neuronal progenitor cells: implications for neuroprotection 

      Koutmani, Y; Politis, P K; Elkouris, M; Agrogiannis, G; Kemerli, M; Patsouris, E; Remboutsika, E; Karalis, K P (Nature Publishing Group, 2013)
      Neurogenesis during embryonic and adult life is tightly regulated by a network of transcriptional, growth and hormonal factors. Emerging evidence indicates that activation of the stress response, via the associated ...
    • Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity 

      Heo, Su-Jin; Driscoll, Tristan P; Thorpe, Stephen D; Nerurkar, Nandan L; Baker, Brendon M; Yang, Michael T; Chen, Christopher S; Lee, David A; Mauck, Robert L (eLife Sciences Publications, Ltd, 2016)
      Mesenchymal stem cell (MSC) differentiation is mediated by soluble and physical cues. In this study, we investigated differentiation-induced transformations in MSC cellular and nuclear biophysical properties and queried ...
    • Directed Differentiation of Pluripotent Stem Cells into Kidney 

      Morizane, Ryuji; Lam, Albert Q (Libertas Academica, 2015)
      Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), represent an ideal substrate for regenerating kidney cells and tissue lost through injury and disease. Recent ...
    • Dorsomorphin, a Selective Small Molecule Inhibitor of BMP Signaling, Promotes Cardiomyogenesis in Embryonic Stem Cells 

      Hao, Jijun; Daleo, Marie A.; Murphy, Clare K.; Yu, Paul B.; Ho, Joshua N.; Hu, Jianyong; Peterson, Randall Theodore; Hatzopoulos, Antonis K.; Hong, Charles C. (Public Library of Science, 2008)
      Background: Pluripotent embryonic stem (ES) cells, which have the capacity to give rise to all tissue types in the body, show great promise as a versatile source of cells for regenerative therapy. However, the basic ...
    • Drop-on-Demand Single Cell Isolation and Total RNA Analysis 

      Moon, Sangjun; Kim, Yun-Gon; Dong, Lingsheng; Lombardi, Michael; Haeggstrom, Edward Olof; Jensen, Roderick V.; Hsiao, Li-Li; Demirci, Utkan (Public Library of Science, 2011)
      Technologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and ...
    • Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder 

      Bavamian, Sabine; Mellios, Nikolaos; Lalonde, Jasmin; Fass, Daniel M.; Wang, Jennifer; Sheridan, Steven D.; Madison, Jon M.; Zhou, Fen; Rueckert, Erroll H.; Barker, Doug; Perlis, Roy H.; Sur, Mriganka; Haggarty, Stephen J. (2014)
      Bipolar disorder (BD) is a heritable neuropsychiatric disorder with largely unknown pathogenesis. Given their prominent role in brain function and disease, we hypothesized that microRNAs (miRNAs) might be of importance for ...
    • Encapsulated stem cells for cancer therapy 

      Shah, Khalid (Landes Bioscience, 2013)
      Stem cells have inherent tumor‑trophic migratory properties and can serve as vehicles for delivering effective, targeted therapy to isolated tumors and metastatic disease, making them promising anti‑cancer agents. Encapsulation ...
    • Enhancer regions show high histone H3.3 turnover that changes during differentiation 

      Deaton, Aimee M; Gómez-Rodríguez, Mariluz; Mieczkowski, Jakub; Tolstorukov, Michael Y; Kundu, Sharmistha; Sadreyev, Ruslan I; Jansen, Lars ET; Kingston, Robert E (eLife Sciences Publications, Ltd, 2016)
      The organization of DNA into chromatin is dynamic; nucleosomes are frequently displaced to facilitate the ability of regulatory proteins to access specific DNA elements. To gain insight into nucleosome dynamics, and to ...
    • Essential role of PU.1 in maintenance of mixed lineage leukemia-associated leukemic stem cells 

      Aikawa, Yukiko; Yamagata, Kazutsune; Katsumoto, Takuo; Shima, Yutaka; Shino, Mika; Stanley, E Richard; Cleary, Michael L; Akashi, Koichi; Tenen, Daniel G; Kitabayashi, Issay (BlackWell Publishing Ltd, 2014)
      Acute myeloid leukemia is a clonal malignant disorder derived from a small number of leukemic stem cells (LSCs). Rearrangements of the mixed lineage leukemia (MLL) gene are found in acute myeloid leukemia associated with ...