Now showing items 1-5 of 5

    • Directed Evolution of Sortase Activity and Specificity 

      Dorr, Brent Matthew (2014-06-06)
      Nature employs complex networks of protein-tailoring enzymes to effect the post-translational modification of proteins in vivo. By comparison, modern chemical methods rely upon either nonspecific labeling techniques or ...
    • Ferristatin II Promotes Degradation of Transferrin Receptor-1 In Vitro and In Vivo 

      Byrne, Shaina L.; Buckett, Peter D.; Kim, Jonghan; Luo, Flora; Sanford, Jack; Chen, Juxing; Enns, Caroline; Wessling-Resnick, Marianne (Public Library of Science, 2013)
      Previous studies have shown that the small molecule iron transport inhibitor ferristatin (NSC30611) acts by down-regulating transferrin receptor-1 (TfR1) via receptor degradation. In this investigation, we show that another ...
    • Generalization of Genetic Code Expansion 

      Stork, Devon (2020-11-23)
      The standard genetic code directs the assembly of the 20 standard amino acids into proteins and defines function in biology. Through the central dogma, DNA is transcribed into RNA which is translated by the well-understood ...
    • Production of α-Galactosylceramide by a Prominent Member of the Human Gut Microbiota 

      Wieland Brown, Laura C.; Penaranda, Cristina; Kashyap, Purna C.; Williams, Brianna B.; Clardy, Jon; Kronenberg, Mitchell; Sonnenburg, Justin L.; Comstock, Laurie E.; Bluestone, Jeffrey A.; Fischbach, Michael A. (Public Library of Science, 2013)
      While the human gut microbiota are suspected to produce diffusible small molecules that modulate host signaling pathways, few of these molecules have been identified. Species of Bacteroides and their relatives, which often ...
    • Proteolysis Controls Endogenous Substance P Levels 

      Mitchell, Andrew J.; Lone, Anna Mari; Tinoco, Arthur D.; Saghatelian, Alan (Public Library of Science, 2013)
      Substance P (SP) is a prototypical neuropeptide with roles in pain and inflammation. Numerous mechanisms regulate endogenous SP levels, including the differential expression of SP mRNA and the controlled secretion of SP ...