Browsing Faculty of Arts and Sciences by FAS Department "Chemical Biology"
Now showing items 1-19 of 19
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Advances in Phage-Assisted Continuous Evolution and Application to Overcoming Bioinsecticide Resistance
(2016-05-20)The Bacillus thuringiensis δ-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits, constituting a substantial and increasingly ... -
All-Optical Neurophysiology in the 1-Photon Regime
(2018-05-13)Simultaneous optical recording and optical stimulation of neuronal activity could enable faster and more comprehensive investigation of neuronal function but is hampered by underdeveloped tools. Pairs of molecular transducers ... -
Assembly and Regulation of the Inflammasome Governed by a Unified Polymerization Mechanism
(2016-05-13)The innate immune system employs a diverse set of pattern recognition receptors to detect intrinsic and extrinsic danger signals for host protection. Inflammasomes represent an important class of receptors that elicit ... -
Biophysical Studies of the Lpt Pathway
(2017-07-06)The outer membrane (OM) of Gram-negative bacteria is impermeable to many antibiotics because its outer leaflet is composed entirely of lipopolysaccharide (LPS), a large glycolipid with an extracellular saccharide that is ... -
The Development of Chemical and Computational Tools to Study Transcriptional Regulation in Cancer
(2015-05-01)Eukaryotic gene regulation is a complex process requiring the action of many multicomponent complexes in the cell. Specific inhibitors of chromatin-associated factors allow the functional study of protein domains without ... -
Directed Evolution and Engineering of CRISPR-Associated Nucleases
(2016-09-12)CRISPR-Cas systems provide prokaryotes with a remarkable mechanism for adaptive immunity, and recent efforts to understand these systems have provided tremendous insight into the complex nature of these systems. Despite ... -
Discovery and Characterization of an Antimicrobial Toxin of the Gut Symbiont Bacteroides fragilis
(2018-08-17)The gut microbiota represents one of most densely populated ecosystems on earth. The trillions of bacteria that populate the gastrointestinal tract carry out essential tasks for the host, such as harvesting energy from ... -
Drugs That Thwart Antibiotic Resistance
(2015-05-20)Antibiotics are often credited with being one of the major forces behind the expansion of human life expectancy in the past 60 years. Yet at the root of this advancement lies its potential undoing: using antibiotics promotes ... -
From Probes to Therapeutics: Chemical Biology Studies of Anaplastic Lymphoma Kinase (ALK) and Cereblon
(2020-01-22)Although there is still discussion surrounding the exact definition of the field, chemical biology is generally described as the development and deployment of a chemical toolbox to manipulate biological systems. The two ... -
Large-Scale Analysis of Microtubule Pharmacology via Phosphoproteomics and High-Content Imaging
(2018-08-17)The dynamic instability of microtubules is a critical phenomenon that regulates several biological processes. Chemical perturbation of microtubules disrupts these processes and is known to stimulate phosphorylation of ... -
Lessons From Cyclosporine A: Structural Determinants of Conformation-Switching and Passive Membrane Penetration
(2015-10-15)The structural complexity of ‘beyond-rule-of-5’ compounds, such as peptide macrocycles, may facilitate access to additional biological target space beyond the enzymatic active site. Naturally occurring cyclic peptides, in ... -
New Genomics Tools and Strategies for Studying Antibiotics and Antibiotic-Resistance in Staphylococcus Aureus
(2016-05-18)Staphylococcus aureus is a gram positive coccoid pathogen that causes intractable infections in hospitals and communities around the world, and tens of thousands of people die of these infections every year. In order to ... -
Phage Assisted Evolutions for Engineering Biosynthetic Pathways
(2018-05-14)Phage Assisted Continuous Evolution (PACE) and its derivative techniques are powerful tools for evolving improved and altered enzymatic activities. Prior to this work, no one had demonstrated the applicability of these ... -
Plasmodium's Crossroads: Deciphering the Molecular Pathway That Leads to Malaria Transmission
(2015-05-13)Plasmodium falciparum is the causative agent of the most severe form of malaria. Transmission from humans to mosquito vectors is an essential step in this eukaryotic parasite‘s life cycle and in the spread of malaria ... -
Reconstitution of the Final Step of Peptidoglycan Assembly in Staphylococcus aureus
(2016-05-10)Bacterial peptidoglycan (PG) is an exoskeleton structure that maintains cell shape and protects cells from lysis. Peptidoglycan is essential in bacteria but is not found in mammalian cells. Therefore, it is the target for ... -
Size Control and Uniformity in Animal Cells
(2015-04-08)The homogeneity in cell size observed in many normal proliferating tissues, and the contrasting size disparities characteristic of several cancers, suggest that control mechanisms coordinate growth and cell cycle progression, ... -
Spatial Organizing Principles of Cytokinesis Signaling
(2015-09-23)Cytokinesis is the final step of cell division, in which the cytoplasm of one cell is physically divided into two. Animal cells integrate multiple positional cues from the mitotic spindle to decide where to position their ... -
The Study of NSD2 Biochemical and Biological Activity Through Small-Molecule Profiling of Cell Lines
(2017-01-18)Histone methyltransferase NSD2 (WHSC1/MMSET) is correlated with several cancers, but promising NSD2 inhibitors are yet to be developed for the clinic, alternative strategies should be studied parallel to probe development ... -
Uncovering Bacterial Metabolites Involved in Eukaryotic Development
(2016-09-09)Microorganisms are ubiquitous. They crowd the soils, the oceans, even deserts and glaciers, and they make up the microbiota that live in and on the bodies of animals. Crammed into their environment with many other competing ...