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Non-Enzymatic Copying of Nucleic Acid Templates
(2014-02-25)
All known living cells contain a complex set of molecular machinery to support their growth and replication. However, the earliest cells must have been much simpler, consisting of a compartment and a genetic material to ...
Investigating Cytoskeletal Motor Mechanisms using DNA Nanotechnology
(2014-02-25)
The microtubule cytoskeleton plays a vital role in the spatial-temporal organization of subcellular cargo required to maintain homeostasis and direct cell division. Cytoplasmic dynein and kinesin are opposite-polarity, ...
Dynamic regulation of histone lysine methylation via the ubiquitin-proteasome system.
(2013-10-18)
Lysine methylation is an important post-translational modification found on histones that is added and removed by histone lysine methyltransferases and demethylases, respectively. Lysine methylation occurs in a specific ...
Deciphering the biological functions of F-box proteins through the use of Parallel Adaptor Capture (PAC) proteomics.
(2013-10-18)
The timely and selective proteasomal degradation of proteins is important for the maintenance of proper cellular processes. Prior to proteasomal degradation, proteins destined for degradation are polyubiquitiylated by ...
Trapping and Manipulating Single Molecules of DNA
(2014-02-25)
This thesis presents the development and application of nanoscale techniques to trap and
Linking Human Genetic Variation to Mitochondrial Metabolism
(2014-06-06)
Genetic variation has a powerful impact on human metabolism and disease. Traditionally, this relationship has either been studied at a high level using top-down descriptive studies of patients with genetically defined ...
The Role of HEXIM1 in the Transcriptional Regulation of Neural Crest Differentiation and Melanoma
(2014-06-06)
Recent evidence suggests that leflunomide, a dihydroorotate dehydrogenase (DHODH) inhibitor, disrupts neural crest development and melanoma pathogenesis via inhibiting transcription elongation. DHODH is an enzyme in the ...
Towards construction of synthetic ribosomes and a self-replicating system
(2014-06-06)
In 2006, the Church Group, using biochemical approaches, hypothesized that ∼ 151 biomolecular components from Escherichia coli and its bacteriophages may be sufficient to enable rapid and accurate self–replication ...
Small-molecule probes to explore cancer
(2014-06-06)
Small molecules play important roles in therapeutics and drug discovery. Significant progress has been made by the chemical biology community to discover small-molecule probes to explore biological processes and to treat ...
Chemical Probes and the Exploration of Bromodomains in Cancer Biology
(2014-06-06)
The post-translational modification of histones and their interaction with transcription factors is essential to gene regulation. Furthermore, these targets would greatly benefit from probe molecules to fully elucidate ...