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Synthetic Genome Construction Techniques to Edit the Genetic Code of Escherichia coli
(2022-11-23)
The expansion of the genetic code to allow for site-specific incorporation of non-standard amino acids (nsAAs) — proteogenic amino acids other than the 22 found across life — is an important step in the development of new ...
Engineering CRISPR-Based DNA Transversion Editing Technologies
(2022-01-11)
Adenine base editors (ABE) and cytosine base editors (CBE) can efficiently install A-to-G and C-to-T transition edits, respectively. However, until recently, the field lacked base editors to efficiently install C-to-G ...
Applications of recombineering and recoded genome construction.
(2022-06-06)
Genome engineering offers the opportunity to bypass natural selection to generate novel organisms. Although multiple types of genome engineering are currently possible, work described in this dissertation occurred in the ...