The expansion of the genetic code to allow for site-specific incorporation of non-standard amino acids (nsAAs) — proteogenic amino acids other than the 22 found across life — is an important step in the development of new ...

The cell envelope surrounds the bacterial cell, providing physical integrity, mediating contact with the surrounding environment, and acting as a barrier against external assaults. The cell wall forms the structural ...

Growth laws emerging from studies of cell populations provide essential constraints on global mechanisms coordinating cell growth. The foundation of bacterial cell cycle studies, based on extensive work done in Escherichia ...

Increasing our understanding of the mechanisms of bacterial cell envelope biogenesis will help identify novel enzymes for future antibiotic targets. While most current research efforts focus on understanding the mechanisms ...

The ability to efficiently and precisely modify the genome of living organisms is one of the most important technological advances of this generation, and propels the accelerating progress of basic research, biotechnology, ...

Tuberculosis (TB) is a leading cause of death globally. Understanding the population dynamics and evolution of TB’s causative agent Mycobacterium tuberculosis complex (MTBC) over short time-scales in-host (micro-evolution) ...

Genome engineering offers the opportunity to bypass natural selection to generate novel organisms. Although multiple types of genome engineering are currently possible, work described in this dissertation occurred in the ...

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis (Mtb), which has been around for millennia and continues to affect millions of people every year. Successful TB treatment requires ...

Although many components of the cell division machinery in bacteria have been identified, the mechanisms by which they work together to divide the cell remain poorly understood. Key among these components is the tubulin ...