Mass Spectrometry-Based Proteomics for Translational Research: A Technical Overview

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Mass Spectrometry-Based Proteomics for Translational Research: A Technical Overview

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Title: Mass Spectrometry-Based Proteomics for Translational Research: A Technical Overview
Author: Kadiyala, Vivek; Paulo, Joao A; Banks, Peter Alan; Steen, Hanno; Conwell, Darwin Lewis

Note: Order does not necessarily reflect citation order of authors.

Citation: Paulo, Joao A., Vivek Kadiyala, Peter A. Banks, Hanno Steen, and Darwin L. Conwell. 2012. Mass spectrometry-based proteomics for translational research: a technical overview. The Yale Journal of Biology and Medicine 85(1): 59-73.
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Abstract: Mass spectrometry-based investigation of clinical samples enables the high-throughput identification of protein biomarkers. We provide an overview of mass spectrometry-based proteomic techniques that are applicable to the investigation of clinical samples. We address sample collection, protein extraction and fractionation, mass spectrometry modalities, and quantitative proteomics. Finally, we examine the limitations and further potential of such technologies. Liquid chromatography fractionation coupled with tandem mass spectrometry is well suited to handle mixtures of hundreds or thousands of proteins. Mass spectrometry-based proteome elucidation can reveal potential biomarkers and aid in the development of hypotheses for downstream investigation of the molecular mechanisms of disease.
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313540/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10060083
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