dc.contributor.author | Chen, Zhihong | |
dc.contributor.author | Ge, Yinlin | |
dc.contributor.author | Kang, Jing Xuan | |
dc.date.accessioned | 2013-01-04T21:03:39Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Chen, Zhihong, Yinlin Ge, and Jing X. Kang. 2004. Down-regulation of the M6P/IGF-II receptor increases cell proliferation and reduces apoptosis in neonatal rat cardiac myocytes. BMC Cell Biology 5:15. | en_US |
dc.identifier.issn | 1471-2121 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10139273 | |
dc.description.abstract | Background: The mannose 6-phosphate/insulin-like growth factor-II receptor (M6P/IGF2R) is a multi-functional protein that has been implicated in regulation of cell growth and apoptosis. Cardiac myocytes express relatively high levels of M6P/IGF2R, and cardiomyocyte apoptosis has been identified in a variety of cardiovascular disorders, such as myocardial infarction and heart failure. However, involvement of M6P/IGF2R in the pathogenesis of these conditions has not been determined. Thus, the objective of this study was to determine the role of M6P/IGF2R in regulation of cardiac myocyte growth and apoptosis. Results: We down-regulated the expression of M6P/IGF2R in neonatal rat cardiac myocytes and examined the effect on cell proliferation and apoptosis. Infection of neonatal cardiomyocytes with an adenovirus expressing a ribozyme targeted against the M6P/IGF2R significantly reduced the level of M6P/IGF2R mRNA, as determined by RT-PCR and Ribonuclease Protection Assay (RPA). M6P-containing protein binding and endocytosis as well as the M6P/IGF2R-mediated internalization of 125I-IGF-II were lower in the ribozyme-treated cells than the control myocytes, indicating that the number of functional M6P/IGF2R in the ribozyme treated cells was reduced. Accordingly, a marked increase in cell proliferation and a reduced cell susceptibility to hypoxia- and TNF-induced apoptosis were observed in the ribozyme-treated cells. Conclusions: These findings suggest that M6P/IGF2R may play a role in regulation of cardiac myocyte growth and apoptosis. Down regulation of this gene in cardiac tissues might be a new approach to prevention of cell death or promotion of mitogenesis for certain heart diseases. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.isversionof | doi://10.1186/1471-2121-5-15 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC411032/pdf/ | en_US |
dc.relation.hasversion | http://www.biomedcentral.com/1471-2121/5/15 | en_US |
dash.license | LAA | |
dc.title | Down-Regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | BMC Cell Biology | en_US |
dash.depositing.author | Kang, Jing Xuan | |
dc.date.available | 2013-01-04T21:03:39Z | |
dash.affiliation.other | HMS^Medicine-Massachusetts General Hospital | en_US |
dc.identifier.doi | 10.1186/1471-2121-5-15 | * |
dash.contributor.affiliated | Kang, Jing | |