A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage
Citation
Ketteler, Robin, Zairen Sun, Karl F. Kovacs, Wei-Wu He, and Brian Seed. 2008. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biology 9(4): R64.Abstract
Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to \(\beta\)-actin. By including protease cleavage sites between GLUC and \(\beta\)-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and \(\beta\)-actin cleavage.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643935/pdf/Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:10140044
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