A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage

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A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage

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Title: A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage
Author: Ketteler, Robin; Sun, Zairen; Kovacs, Karl F; He, Wei-Wu; Seed, Brian

Note: Order does not necessarily reflect citation order of authors.

Citation: Ketteler, Robin, Zairen Sun, Karl F. Kovacs, Wei-Wu He, and Brian Seed. 2008. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biology 9(4): R64.
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Abstract: Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to \(\beta\)-actin. By including protease cleavage sites between GLUC and \(\beta\)-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and \(\beta\)-actin cleavage.
Published Version: doi://10.1186/gb-2008-9-4-r64
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643935/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10140044
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