A Pathway Sensor for Genome-Wide Screens of Intracellular Proteolytic Cleavage
Kovacs, Karl F
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CitationKetteler, Robin, Zairen Sun, Karl F. Kovacs, Wei-Wu He, and Brian Seed. 2008. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage. Genome Biology 9(4): R64.
AbstractProtein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to \(\beta\)-actin. By including protease cleavage sites between GLUC and \(\beta\)-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of autophagy, apoptosis and \(\beta\)-actin cleavage.
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