Thin Sectioning of Slice Preparations for Immunohistochemistry

DSpace/Manakin Repository

Thin Sectioning of Slice Preparations for Immunohistochemistry

Citable link to this page

 

 
Title: Thin Sectioning of Slice Preparations for Immunohistochemistry
Author: Park, Jae-Joon; Cunningham, Miles Gregory

Note: Order does not necessarily reflect citation order of authors.

Citation: Park, Jae-Joon, and Miles G. Cunningham. 2007. Thin sectioning of slice preparations for immunohistochemistry. Journal of Visualized Experiments 3:e194.
Full Text & Related Files:
Abstract: Many investigations in neuroscience, as well as other disciplines, involve studying small, yet macroscopic pieces or sections of tissue that have been preserved, freshly removed, or excised but kept viable, as in slice preparations of brain tissue. Subsequent microscopic studies of this material can be challenging, as the tissue samples may be difficult to handle. Demonstrated here is a method for obtaining thin cryostat sections of tissue with a thickness that may range from 0.2-5.0 mm. We routinely cut 400 micron thick Vibratome brain slices serially into 5-10 micron coronal cryostat sections. The slices are typically first used for electrophysiology experiments and then require microscopic analysis of the cytoarchitecture of the region from which the recordings were observed. We have constructed a simple device that allows controlled and reproducible preparation and positioning of the tissue slice. This device consists of a cylinder 5 cm in length with a diameter of 1.2 cm, which serves as a freezing stage for the slice. A ring snugly slides over the cylinder providing walls around the slice allowing the tissue to be immersed in freezing compound (e.g., OCT). This is then quickly frozen with crushed dry ice and the resulting wafer can be position easily for cryostat sectioning. Thin sections can be thaw-mounted onto coated slides to allow further studies to be performed, such as various staining methods, in situ hybridization, or immunohistochemistry, as demonstrated here.
Published Version: doi://10.3791/194
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2535837/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10178132
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters