DNA Dynamics Play a Role as a Basal Transcription Factor in the Positioning and Regulation of Gene Transcription Initiation

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DNA Dynamics Play a Role as a Basal Transcription Factor in the Positioning and Regulation of Gene Transcription Initiation

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Title: DNA Dynamics Play a Role as a Basal Transcription Factor in the Positioning and Regulation of Gene Transcription Initiation
Author: Alexandrov, Boian S.; Alexandrov, Ludmil B.; Fukuyo, Yayoi; Bishop, Alan R.; Rasmussen, Kim Ø.; Usheva, Anny; Gelev, Vladimir; Yoo, Sang Wook

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Citation: Alexandrov, Boian S., Vladimir Gelev, Sang Wook Yoo, Ludmil B. Alexandrov, Yayoi Fukuyo, Alan R. Bishop, Kim Ø. Rasmussen, and Anny Usheva. 2010. DNA dynamics play a role as a basal transcription factor in the positioning and regulation of gene transcription initiation. Nucleic Acids Research 38(6): 1790-1795.
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Abstract: We assess the role of DNA breathing dynamics as a determinant of promoter strength and transcription start site (TSS) location. We compare DNA Langevin dynamic profiles of representative gene promoters, calculated with the extended non-linear PBD model of DNA with experimental data on transcription factor binding and transcriptional activity. Our results demonstrate that DNA dynamic activity at the TSS can be suppressed by mutations that do not affect basal transcription factor binding–DNA contacts. We use this effect to establish the separate contributions of transcription factor binding and DNA dynamics to transcriptional activity. Our results argue against a purely ‘transcription factor-centric’ view of transcription initiation, suggesting that both DNA dynamics and transcription factor binding are necessary conditions for transcription initiation.
Published Version: doi://10.1093/nar/gkp1084
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847213/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10178138
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