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dc.contributor.authorAlexandrov, Boian S.
dc.contributor.authorAlexandrov, Ludmil B.
dc.contributor.authorFukuyo, Yayoi
dc.contributor.authorBishop, Alan R.
dc.contributor.authorRasmussen, Kim Ø.
dc.contributor.authorUsheva, Anny
dc.contributor.authorGelev, Vladimir
dc.contributor.authorYoo, Sang Wook
dc.date.accessioned2013-01-15T16:51:39Z
dc.date.issued2009
dc.identifier.citationAlexandrov, Boian S., Vladimir Gelev, Sang Wook Yoo, Ludmil B. Alexandrov, Yayoi Fukuyo, Alan R. Bishop, Kim Ø. Rasmussen, and Anny Usheva. 2010. DNA dynamics play a role as a basal transcription factor in the positioning and regulation of gene transcription initiation. Nucleic Acids Research 38(6): 1790-1795.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10178138
dc.description.abstractWe assess the role of DNA breathing dynamics as a determinant of promoter strength and transcription start site (TSS) location. We compare DNA Langevin dynamic profiles of representative gene promoters, calculated with the extended non-linear PBD model of DNA with experimental data on transcription factor binding and transcriptional activity. Our results demonstrate that DNA dynamic activity at the TSS can be suppressed by mutations that do not affect basal transcription factor binding–DNA contacts. We use this effect to establish the separate contributions of transcription factor binding and DNA dynamics to transcriptional activity. Our results argue against a purely ‘transcription factor-centric’ view of transcription initiation, suggesting that both DNA dynamics and transcription factor binding are necessary conditions for transcription initiation.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofdoi://10.1093/nar/gkp1084en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847213/pdf/en_US
dash.licenseLAA
dc.titleDNA Dynamics Play a Role as a Basal Transcription Factor in the Positioning and Regulation of Gene Transcription Initiationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNucleic Acids Researchen_US
dash.depositing.authorGelev, Vladimir
dc.date.available2013-01-15T16:51:39Z
dash.affiliation.otherHMS^Obstetrics Gynecology and Repro. Bio. - BIDMCen_US
dc.identifier.doi10.1093/nar/gkp1084*
dash.authorsorderedfalse
dash.contributor.affiliatedGelev, Vladimir
dash.contributor.affiliatedYoo, Sang Wook


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