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dc.contributor.authorBandyopadhyay, Amitabha
dc.contributor.authorTsuji, Kunikazu
dc.contributor.authorCox, Karen Ann
dc.contributor.authorHarfe, Brian D
dc.contributor.authorRosen, Vicki
dc.contributor.authorTabin, Clifford James
dc.date.accessioned2013-01-15T17:14:43Z
dc.date.issued2006
dc.identifier.citationBandyopadhyay, Amitabha, Kunikazu Tsuji, Karen Ann Cox, Brian D. Harfe, Vicki Rosen, and Clifford James Tabin. 2006. Genetic analysis of the roles of BMP2, BMP4, and BMP7 in limb patterning and skeletogenesis. PLoS Genetics 2(12): e216.en_US
dc.identifier.issn1553-7390en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10178301
dc.description.abstractBone morphogenetic protein (BMP) family members, including BMP2, BMP4, and BMP7, are expressed throughout limb development. BMPs have been implicated in early limb patterning as well as in the process of skeletogenesis. However, due to complications associated with early embryonic lethality, particularly for Bmp2 and Bmp4, and with functional redundancy among BMP molecules, it has been difficult to decipher the specific roles of these BMP molecules during different stages of limb development. To circumvent these issues, we have constructed a series of mouse strains lacking one or more of these BMPs, using conditional alleles in the case of Bmp2 and Bmp4 to remove them specifically from the limb bud mesenchyme. Contrary to earlier suggestions, our results indicate that BMPs neither act as secondary signals downstream of Sonic Hedghog (SHH) in patterning the anteroposterior axis nor as signals from the interdigital mesenchyme in specifying digit identity. We do find that a threshold level of BMP signaling is required for the onset of chondrogenesis, and hence some chondrogenic condensations fail to form in limbs deficient in both BMP2 and BMP4. However, in the condensations that do form, subsequent chondrogenic differentiation proceeds normally even in the absence of BMP2 and BMP7 or BMP2 and BMP4. In contrast, we find that the loss of both BMP2 and BMP4 results in a severe impairment of osteogenesis.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pgen.0020216en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1713256/pdf/en_US
dash.licenseLAA
dc.titleGenetic Analysis of the Roles of BMP2, BMP4, and BMP7 in Limb Patterning and Skeletogenesisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Geneticsen_US
dash.depositing.authorTabin, Clifford James
dc.date.available2013-01-15T17:14:43Z
dash.affiliation.otherHMS^Health Sciences and Technologyen_US
dash.affiliation.otherHMS^Geneticsen_US
dc.identifier.doi10.1371/journal.pgen.0020216*
dash.contributor.affiliatedRosen, Vicki
dash.contributor.affiliatedCox, Karen
dash.contributor.affiliatedTabin, Clifford


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