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dc.contributor.authorSantagata, Sandro
dc.contributor.authorMaire, Cecile
dc.contributor.authorIdbaih, Ahmed
dc.contributor.authorGeffers, Lars
dc.contributor.authorCorrell, Mick
dc.contributor.authorHolton, Kristina
dc.contributor.authorQuackenbush, John
dc.contributor.authorLigon, Keith Lloyd
dc.date.accessioned2013-01-18T19:04:24Z
dc.date.issued2009
dc.identifier.citationSantagata, Sandro, Cecile L. Maire, Ahmed Idbaih, Lars Geffers, Mick Correll, Kristina Holton, John Quackenbush, and Keith L. Ligon. 2009. CRX is a diagnostic marker of retinal and pineal lineage tumors. PLoS ONE 4(11): e7932.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10198690
dc.description.abstractBackground: CRX is a homeobox transcription factor whose expression and function is critical to maintain retinal and pineal lineage cells and their progenitors. To determine the biologic and diagnostic potential of CRX in human tumors of the retina and pineal, we examined its expression in multiple settings. Methodology/Principal Findings: Using situ hybridization and immunohistochemistry we show that Crx RNA and protein expression are exquisitely lineage restricted to retinal and pineal cells during normal mouse and human development. Gene expression profiling analysis of a wide range of human cancers and cancer cell lines also supports that CRX RNA is highly lineage restricted in cancer. Immunohistochemical analysis of 22 retinoblastomas and 13 pineal parenchymal tumors demonstrated strong expression of CRX in over 95% of these tumors. Importantly, CRX was not detected in the majority of tumors considered in the differential diagnosis of pineal region tumors (n = 78). The notable exception was medulloblastoma, 40% of which exhibited CRX expression in a heterogeneous pattern readily distinguished from that seen in retino-pineal tumors. Conclusions/Significance: These findings describe new potential roles for CRX in human cancers and highlight the general utility of lineage restricted transcription factors in cancer biology. They also identify CRX as a sensitive and specific clinical marker and a potential lineage dependent therapeutic target in retinoblastoma and pineoblastoma.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi://10.1371/journal.pone.0007932en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775954/pdf/en_US
dash.licenseLAA
dc.subjectdevelopmental biologyen_US
dc.subjectneurodevelopmenten_US
dc.subjectneurological disordersen_US
dc.subjectneuroendocrinologyen_US
dc.subjectneuro-oncologyen_US
dc.subjectoncologyen_US
dc.subjectpediatric oncologyen_US
dc.subjectpathologyen_US
dc.subjectneuropathologyen_US
dc.titleCRX is a Diagnostic Marker of Retinal and Pineal Lineage Tumorsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorSantagata, Sandro
dc.date.available2013-01-18T19:04:24Z
dash.affiliation.otherHMS^Pathologyen_US
dc.identifier.doi10.1371/journal.pone.0007932*
dash.contributor.affiliatedMaire, Cecile
dash.contributor.affiliatedQuackenbush, John
dash.contributor.affiliatedSantagata, Sandro
dash.contributor.affiliatedLigon, Keith
dc.identifier.orcid0000-0002-2702-5879


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