Viral Capsid Is a Pathogen-Associated Molecular Pattern in Adenovirus Keratitis

DSpace/Manakin Repository

Viral Capsid Is a Pathogen-Associated Molecular Pattern in Adenovirus Keratitis

Citable link to this page

 

 
Title: Viral Capsid Is a Pathogen-Associated Molecular Pattern in Adenovirus Keratitis
Author: Chintakuntlawar, Ashish V.; Rajaiya, Jayabarathy; Chodosh, James; Zhou, Xiaohong

Note: Order does not necessarily reflect citation order of authors.

Citation: Chintakuntlawar, Ashish V., Xiaohong Zhou, Jaya Rajaiya, and James Chodosh. 2010. Viral Capsid Is a Pathogen-Associated Molecular Pattern in Adenovirus Keratitis. PLoS Pathogens 6(4): e1000841.
Full Text & Related Files:
Abstract: Human adenovirus (HAdV) infection of the human eye, in particular serotypes 8, 19 and 37, induces the formation of corneal subepithelial leukocytic infiltrates. Using a unique mouse model of adenovirus keratitis, we studied the role of various virus-associated molecular patterns in subsequent innate immune responses of resident corneal cells to HAdV-37 infection. We found that neither viral DNA, viral gene expression, or viral replication was necessary for the development of keratitis. In contrast, empty viral capsid induced keratitis and a chemokine profile similar to intact virus. Transfected viral DNA did not induce leukocyte infiltration despite CCL2 expression similar to levels in virus infected corneas. Mice without toll-like receptor 9 (Tlr9) signaling developed clinical keratitis upon HAdV-37 infection similar to wild type mice, although the absolute numbers of activated monocytes in the cornea were less in Tlr9\(^{−/−}\) mice. Virus induced leukocytic infiltrates and chemokine expression in mouse cornea could be blocked by treatment with a peptide containing arginine glycine aspartic acid (RGD). These results demonstrate that adenovirus infection of the cornea induces chemokine expression and subsequent infiltration by leukocytes principally through RGD contact between viral capsid and the host cell, possibly through direct interaction between the viral capsid penton base and host cell integrins.
Published Version: doi://10.1371/journal.ppat.1000841
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2855317/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10198958
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters