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dc.contributor.authorSander, Jeffry D
dc.contributor.authorZaback, Peter
dc.contributor.authorJoung, Jae Keith
dc.contributor.authorVoytas, Daniel F.
dc.contributor.authorDobbs, Drena
dc.date.accessioned2013-01-30T19:30:49Z
dc.date.issued2007
dc.identifier.citationSander, Jeffry D., Peter Zaback, J. Keith Joung, Daniel F. Voytas, and Drena Dobbs. 2007. Zinc Finger Targeter (ZiFiT): An engineered zinc finger/target site design tool. Nucleic Acids Research 35(Suppl. 2): W599-W605.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10246886
dc.description.abstractZinc Finger Targeter (ZiFiT) is a simple and intuitive web-based tool that facilitates the design of zinc finger proteins (ZFPs) that can bind to specific DNA sequences. The current version of ZiFiT is based on a widely employed method of ZFP design, the ‘modular assembly’ approach, in which pre-existing individual zinc fingers are linked together to recognize desired target DNA sequences. Several research groups have described experimentally characterized zinc finger modules that bind many of the 64 possible DNA triplets. ZiFiT leverages the combined capabilities of three of the largest and best characterized module archives by enabling users to select fingers from any of these sets. ZiFiT searches a query DNA sequence for target sites for which a ZFP can be designed using modules available in one or more of the three archives. In addition, ZiFiT output facilitates identification of specific zinc finger modules that are publicly available from the Zinc Finger Consortium. ZiFiT is freely available at http://bindr.gdcb.iastate.edu/ZiFiT/.en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofdoi://10.1093/nar/gkm349en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933188/pdf/en_US
dash.licenseLAA
dc.titleZinc Finger Targeter (ZiFiT): An Engineered Zinc Finger/Target Site Design Toolen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNucleic Acids Researchen_US
dash.depositing.authorJoung, Jae Keith
dc.date.available2013-01-30T19:30:49Z
dash.affiliation.otherHMS^Pathologyen_US
dc.identifier.doi10.1093/nar/gkm349*
dash.contributor.affiliatedSander, Jeffry D.
dash.contributor.affiliatedJoung, Keith


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