SIRT1 mRNA Expression May Be Associated With Energy Expenditure and Insulin Sensitivity

DSpace/Manakin Repository

SIRT1 mRNA Expression May Be Associated With Energy Expenditure and Insulin Sensitivity

Citable link to this page

 

 
Title: SIRT1 mRNA Expression May Be Associated With Energy Expenditure and Insulin Sensitivity
Author: Rutanen, Jarno; Yaluri, Nagendra; Modi, Shalem; Itkonen, Paula; Kainulainen, Sakari; Yamamoto, Hiroyasu; Lagouge, Marie; Elliott, Peter; Westphal, Christoph; Auwerx, Johan; Laakso, Markku; Sinclair, David Andrew; Vänttinen, Markku; Pihlajamäki, Jussi

Note: Order does not necessarily reflect citation order of authors.

Citation: Rutanen, Jarno, Nagendra Yaluri, Shalem Modi, Jussi Pihlajamäki, Markku Vänttinen, Paula Itkonen, David A. Sinclair, et al. 2010. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity. Diabetes 59(4): 829-835.
Full Text & Related Files:
Abstract: Objective: Sirtuin 1 (SIRT1) is implicated in the regulation of mitochondrial function, energy metabolism, and insulin sensitivity in rodents. No studies are available in humans to demonstrate that SIRT1 expression in insulin-sensitive tissues is associated with energy expenditure and insulin sensitivity. Research Design and Methods: Energy expenditure (EE), insulin sensitivity, and SIRT1 mRNA adipose tissue expression (n = 81) were measured by indirect calorimetry, hyperinsulinemic-euglycemic clamp, and quantitative RT-PCR in 247 nondiabetic offspring of type 2 diabetic patients. Results: High EE during the clamp (r = 0.375, P = 2.8 × 10\(^{−9}\)) and high \(\Delta\)EE (EE during the clamp − EE in the fasting state) (r = 0.602, P = 2.5 × 10\(^{−24}\)) were associated with high insulin sensitivity. Adipose tissue SIRT1 mRNA expression was significantly associated with EE (r = 0.289, P = 0.010) and with insulin sensitivity (r = 0.334, P = 0.002) during hyperinsulinemic-euglycemic clamp. Furthermore, SIRT1 mRNA expression correlated significantly with the expression of several genes regulating mitochondrial function and energy metabolism (e.g., peroxisome proliferator–activated receptor \(\gamma\) coactivator-1\(\beta\), estrogen-related receptor \(\alpha\), nuclear respiratory factor-1, and mitochondrial transcription factor A), and with several genes of the respiratory chain (e.g., including NADH dehydrogenase [ubiquinone] 1\(\alpha\) subcomplex 2, cytochrome c, cytochrome c oxidase subunit IV, and ATP synthase). Conclusions: Impaired stimulation of EE by insulin and low SIRT1 expression in insulin-sensitive tissues is likely to reflect impaired regulation of mitochondrial function associated with insulin resistance in humans.
Published Version: doi://10.2337/db09-1191
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2844830/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10251496
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters