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dc.contributor.authorVinegoni, Claudio
dc.contributor.authorRazansky, Daniel
dc.contributor.authorFigueiredo, Jose-Luiz
dc.contributor.authorFexon, Lyuba
dc.contributor.authorPivovarov, Misha
dc.contributor.authorNahrendorf, Matthias
dc.contributor.authorNtziachristos, Vasilis
dc.contributor.authorWeissleder, Ralph
dc.date.accessioned2013-02-04T18:46:40Z
dc.date.issued2009
dc.identifier.citationVinegoni, Claudio, Daniel Razansky, Jose-Luiz Figueiredo, Lyuba Fexon, Misha Pivovarov, Matthias Nahrendorf, Vasilis Ntziachristos, and Ralph Weissleder. 2009. Born normalization for fluorescence optical projection tomography for whole heart imaging. Journal of Visualized Experiments 28: e1389.en_US
dc.identifier.issn1940-087Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10265020
dc.description.abstractOptical projection tomography is a three-dimensional imaging technique that has been recently introduced as an imaging tool primarily in developmental biology and gene expression studies. The technique renders biological sample optically transparent by first dehydrating them and then placing in a mixture of benzyl alcohol and benzyl benzoate in a 2:1 ratio (BABB or Murray s Clear solution). The technique renders biological samples optically transparent by first dehydrating them in graded ethanol solutions then placing them in a mixture of benzyl alcohol and benzyl benzoate in a 2:1 ratio (BABB or Murray s Clear solution) to clear. After the clearing process the scattering contribution in the sample can be greatly reduced and made almost negligible while the absorption contribution cannot be eliminated completely. When trying to reconstruct the fluorescence distribution within the sample under investigation, this contribution affects the reconstructions and leads, inevitably, to image artifacts and quantification errors.. While absorption could be reduced further with a permanence of weeks or months in the clearing media, this will lead to progressive loss of fluorescence and to an unrealistically long sample processing time. This is true when reconstructing both exogenous contrast agents (molecular contrast agents) as well as endogenous contrast (e.g. reconstructions of genetically expressed fluorescent proteins).en_US
dc.language.isoen_USen_US
dc.publisherMyJove Corporationen_US
dc.relation.isversionofdoi://10.3791/1389en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794886/pdf/en_US
dash.licenseLAA
dc.subjectbioengineeringen_US
dc.subjectoptical imagingen_US
dc.subjectfluorescence imagingen_US
dc.subjectoptical projection tomographyen_US
dc.subjectborn normalizationen_US
dc.subjectmolecular imagingen_US
dc.titleBorn Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imagingen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Visualized Experimentsen_US
dash.depositing.authorWeissleder, Ralph
dc.date.available2013-02-04T18:46:40Z
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Radiology-Massachusetts General Hospitalen_US
dash.affiliation.otherHMS^Systems Biologyen_US
dc.identifier.doi10.3791/1389*
dash.contributor.affiliatedVinegoni, Claudio
dash.contributor.affiliatedPivovarov, Misha
dash.contributor.affiliatedNahrendorf, Matthias
dash.contributor.affiliatedWeissleder, Ralph


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