Two Classes of Silencing RNAs Move between Caenorhabditis elegans Tissues

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Two Classes of Silencing RNAs Move between Caenorhabditis elegans Tissues

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Title: Two Classes of Silencing RNAs Move between Caenorhabditis elegans Tissues
Author: Jose, Antony Merlin; Garcia, Giancarlo; Hunter, Craig P.

Note: Order does not necessarily reflect citation order of authors.

Citation: Jose, Antony M., Giancarlo A. Garcia, and Craig P. Hunter. 2011. Two classes of silencing RNAs move between Caenorhabditis elegans tissues. Nature Structural & Molecular Biology 18(11): 1184-1188.
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Abstract: Organism-wide RNA interference (RNAi) is due to the transport of mobile silencing RNA throughout the organism, but the identities of these mobile RNA species in animals are unknown. Here, we present genetic evidence that both the initial double-stranded RNA (dsRNA), which triggers RNAi, and at least one dsRNA intermediate produced during RNAi can act as or generate mobile silencing RNA in C. elegans. This dsRNA intermediate requires the long dsRNA-binding protein RDE-4, the endonuclease DCR-1, which cleaves long dsRNA into double-stranded short-interfering RNA (ds-siRNA), and the putative nucleotidyltransferase MUT-2 (RDE-3). However, single-stranded siRNA and downstream secondary siRNA produced upon amplification by the RNA-dependent RNA polymerase RRF-1 do not generate mobile silencing RNA. Restricting intertissue transport to long dsRNA and directly processed siRNA intermediates rather than amplified siRNA may serve to modulate the extent of systemic silencing in proportion to available dsRNA.
Published Version: doi:10.1038/nsmb.2134
Other Sources: http://www.clfs.umd.edu/cbmg/joselab/myPdfs/JoseEtAl_NSMB_2011.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10265439
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