Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes

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Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes

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Title: Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes
Author: Costantino, Cristina Maria; Spooner, Eric; Ploegh, Hidde L.; Hafler, David A.

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Citation: Costantino, Cristina Maria, Eric Spooner, Hidde L. Ploegh, and David A. Hafler. 2012. Class II MHC self-antigen presentation in human B and T lymphocytes. PLoS ONE 7(1): e29805.
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Abstract: Human CD4\(^+\) T cells process and present functional class II MHC-peptide complexes, but the endogenous peptide repertoire of these non-classical antigen presenting cells remains unknown. We eluted and sequenced HLA-DR-bound self-peptides presented by CD4\(^+\) T cells in order to compare the T cell-derived peptide repertoire to sequences derived from genetically identical B cells. We identified several novel epitopes derived from the T cell-specific proteome, including fragments of CD4 and IL-2. While these data confirm that T cells can present peptides derived from the T-cell specific proteome, the vast majority of peptides sequenced after elution from MHC were derived from the common proteome. From this pool, we identified several identical peptide epitopes in the T and B cell repertoire derived from common endogenous proteins as well as novel endogenous epitopes with promiscuous binding. These findings indicate that the endogenous HLA-DR-bound peptide repertoire, regardless of APC type and across MHC isotype, is largely derived from the same pool of self-protein.
Published Version: doi://10.1371/journal.pone.0029805
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