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dc.contributor.authorFujiwara, Kazushiro
dc.contributor.authorHasegawa, Koichi
dc.contributor.authorOhkumo, Tsuyoshi
dc.contributor.authorMiyoshi, Hiroyuki
dc.contributor.authorTseng, Yu-Hua
dc.contributor.authorYoshikawa, Kazuaki
dc.date.accessioned2013-02-13T18:22:09Z
dc.date.issued2012
dc.identifier.citationFujiwara, Kazushiro, Koichi Hasegawa, Tsuyoshi Ohkumo, Hiroyuki Miyoshi, Yu-Hua Tseng, and Kazuaki Yoshikawa. 2012. Necdin controls proliferation of white adipocyte progenitor cells. PLoS ONE 7(1): e30948.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10288817
dc.description.abstractWhite adipose tissues are composed mainly of white fat cells (adipocytes), which play a key role in energy storage and metabolism. White adipocytes are terminally differentiated postmitotic cells and arise from their progenitor cells (preadipocytes) or mesenchymal stem cells residing in white adipose tissues. Thus, white adipocyte number is most likely controlled by the rate of preadipocyte proliferation, which may contribute to the etiology of obesity. However, little is known about the molecular mechanisms that regulate preadipocyte proliferation during adipose tissue development. Necdin, which is expressed predominantly in postmitotic neurons, is a pleiotropic protein that possesses anti-mitotic and pro-survival activities. Here we show that necdin functions as an intrinsic regulator of white preadipocyte proliferation in developing adipose tissues. Necdin is expressed in early preadipocytes or mesenchymal stem cells residing in the stromal compartment of white adipose tissues in juvenile mice. Lentivirus-mediated knockdown of endogenous necdin expression in vivo in adipose tissues markedly increases fat mass in juvenile mice fed a high-fat diet until adulthood. Furthermore, necdin-null mutant mice exhibit a greater expansion of adipose tissues due to adipocyte hyperplasia than wild-type mice when fed the high-fat diet during the juvenile and adult periods. Adipose stromal-vascular cells prepared from necdin-null mice differentiate in vitro into a significantly larger number of adipocytes in response to adipogenic inducers than those from wild-type mice. These results suggest that necdin prevents excessive preadipocyte proliferation induced by adipogenic stimulation to control white adipocyte number during adipose tissue development.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0030948en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264651/pdf/en_US
dash.licenseLAA
dc.subjectbiologyen_US
dc.subjectcomputational biologyen_US
dc.subjectmolecular geneticsen_US
dc.subjectdevelopmental biologyen_US
dc.subjectgeneticsen_US
dc.subjectmolecular cell biologyen_US
dc.titleNecdin Controls Proliferation of White Adipocyte Progenitor Cellsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorTseng, Yu-Hua
dc.date.available2013-02-13T18:22:09Z
dc.identifier.doi10.1371/journal.pone.0030948*
dash.contributor.affiliatedTseng, Yu-Hua


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