Jetset: Selecting the Optimal Microarray Probe Set to Represent a Gene

DSpace/Manakin Repository

Jetset: Selecting the Optimal Microarray Probe Set to Represent a Gene

Citable link to this page


Title: Jetset: Selecting the Optimal Microarray Probe Set to Represent a Gene
Author: Gyorffy, Balazs; Eklund, Aron C; Li, Qiyuan; Birkbak, Nicolai Juul; Szallasi, Zoltan

Note: Order does not necessarily reflect citation order of authors.

Citation: Li, Qiyuan, Nicolai J. Birkbak, Balazs Gyorffy, Zoltan Szallasi, and Aron C. Eklund. 2011. Jetset: Selecting the optimal microarray probe set to represent a gene. BMC Bioinformatics 12:474.
Full Text & Related Files:
Abstract: Background: Interpretation of gene expression microarrays requires a mapping from probe set to gene. On many Affymetrix gene expression microarrays, a given gene may be detected by multiple probe sets, which may deliver inconsistent or even contradictory measurements. Therefore, obtaining an unambiguous expression estimate of a pre-specified gene can be a nontrivial but essential task. Results: We developed scoring methods to assess each probe set for specificity, splice isoform coverage, and robustness against transcript degradation. We used these scores to select a single representative probe set for each gene, thus creating a simple one-to-one mapping between gene and probe set. To test this method, we evaluated concordance between protein measurements and gene expression values, and between sets of genes whose expression is known to be correlated. For both test cases, we identified genes that were nominally detected by multiple probe sets, and we found that the probe set chosen by our method showed stronger concordance. Conclusions: This method provides a simple, unambiguous mapping to allow assessment of the expression levels of specific genes of interest.
Published Version: doi:10.1186/1471-2105-12-474
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search