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dc.contributor.authorHabibollahi, Peiman
dc.contributor.authorFigueiredo, Jose-Luiz
dc.contributor.authorHeidari, Pedram
dc.contributor.authorDulak, Austin M
dc.contributor.authorImamura, Yu
dc.contributor.authorBass, Adam Joel
dc.contributor.authorOgino, Shuji
dc.contributor.authorChan, Andrew Tan
dc.contributor.authorMahmood, Umar
dc.date.accessioned2013-02-15T20:02:44Z
dc.date.issued2012
dc.identifier.citationHabibollahi, Peiman, Jose-Luiz Figueiredo, Pedram Heidari, Austin M. Dulak, Yu Imamura, Adam J. Bass, Shuji Ogino, Andrew T. Chan, and Umar Mahmood. 2012. Optical imaging with a cathepsin B activated probe for the enhanced detection of esophageal adenocarcinoma by dual channel fluorescent upper GI endoscopy. Theranostics 2(2): 227-234.en_US
dc.identifier.issn1838-7640en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10304413
dc.description.abstractDespite significant advances in diagnosis and treatment, the prognosis of esophageal adenocarcinoma remains poor highlighting the importance of early detection. Although white light (WL) upper endoscopy can be used for screening of the esophagus, it has limited sensitivity for early stage disease. Thus, development of new imaging technology to improve the diagnostic capabilities of upper GI endoscopy for early detection of esophageal adenocarcinoma is an important unmet need. The goal of this study was to develop a method for the detection of malignant lesions in the esophagus using WL upper endoscopy combined with near infrared (NIR) imaging with a protease activatable probe (Prosense750) selective for cathepsin B (CTSB). An orthotopic murine model for distal esophageal adenocarcinoma was generated through the implantation of OE-33 and OE-19 human esophageal adenocarcinoma lines in immunocompromised mice. The mice were imaged simultaneously for WL and NIR signal using a custom-built dual channel upper GI endoscope. The presence of tumor was confirmed by histology and target to background ratios (TBR) were compared for both WL and NIR imaging. NIR imaging with ProSense750 significantly improved upon the TBRs of esophageal tumor foci, with a TBR of 3.64\(\pm\)0.14 and 4.50\(\pm\)0.11 for the OE-33 and OE-19 tumors respectively, compared to 0.88\(\pm\)0.04 and 0.81\(\pm\)0.02 TBR for WL imaging. The combination of protease probes with novel imaging devices has the potential to improve esophageal tumor detection by fluorescently highlighting neoplastic regions.en_US
dc.language.isoen_USen_US
dc.publisherIvyspring International Publisheren_US
dc.relation.isversionofdoi:10.7150/thno.4088en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296470/pdf/en_US
dash.licenseLAA
dc.titleOptical Imaging with a Cathepsin B Activated Probe for the Enhanced Detection of Esophageal Adenocarcinoma by Dual Channel Fluorescent Upper GI Endoscopyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalTheranosticsen_US
dash.depositing.authorBass, Adam Joel
dc.date.available2013-02-15T20:02:44Z
dc.identifier.doi10.7150/thno.4088*
dash.contributor.affiliatedImamura, Yu
dash.contributor.affiliatedMahmood, Umar
dash.contributor.affiliatedChan, Andrew
dash.contributor.affiliatedBass, Adam
dash.contributor.affiliatedHeidari, Pedram
dash.contributor.affiliatedOgino, Shuji


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