Computational and Serologic Analysis of Novel and Known Viruses in Species Human Adenovirus D in Which Serology and Genomics Do Not Correlate
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Author
Liu, Elizabeth B.
Wadford, Debra A.
Seto, Jason
Vu, Maria
Hudson, Nolan Ryan
Thrasher, Lisa
Torres, Sarah
Dyer, David W.
Seto, Donald
Jones, Morris S.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1371/journal.pone.0033212Metadata
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Liu, Elizabeth B., Debra A. Wadford, Jason Seto, Maria Vu, Nolan Ryan Hudson, Lisa Thrasher, Sarah Torres, et al. 2012. Computational and serologic analysis of novel and known viruses in species human adenovirus D in which serology and genomics do not correlate. PLoS ONE 7(3): e33212.Abstract
In November of 2007 a human adenovirus (HAdV) was isolated from a bronchoalveolar lavage (BAL) sample recovered from a biopsy of an AIDS patient who presented with fever, cough, tachycardia, and expiratory wheezes. To better understand the isolated virus, the genome was sequenced and analyzed using bioinformatic and phylogenomic analysis. The results suggest that this novel virus, which is provisionally named HAdV-D59, may have been created from multiple recombination events. Specifically, the penton, hexon, and fiber genes have high nucleotide identity to HAdV-D19C, HAdV-D25, and HAdV-D56, respectively. Serological results demonstrated that HAdV-D59 has a neutralization profile that is similar yet not identical to that of HAdV-D25. Furthermore, we observed a two-fold difference between the ability of HAdV-D15 and HAdV-D25 to be neutralized by reciprocal antiserum indicating that the two hexon proteins may be more similar in epitopic conformation than previously assumed. In contrast, hexon loops 1 and 2 of HAdV-D15 and HAdV-D25 share 79.13 and 92.56 percent nucleotide identity, respectively. These data suggest that serology and genomics do not always correlate.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302849/pdf/Terms of Use
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