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dc.contributor.authorRattanama, Pimonsri
dc.contributor.authorThompson, Janelle R.
dc.contributor.authorKongkerd, Natthawan
dc.contributor.authorSrinitiwarawong, Kanchana
dc.contributor.authorVuddhakul, Varaporn
dc.contributor.authorMekalanos, John J.
dc.date.accessioned2013-02-21T18:27:33Z
dc.date.issued2012
dc.identifier.citationRattanama, Pimonsri, Janelle R. Thompson, Natthawan Kongkerd, Kanchana Srinitiwarawong, Varaporn Vuddhakul, and John J. Mekalanos. 2012. Sigma E regulators control hemolytic activity and virulence in a shrimp pathogenic vibrio harveyi. PLoS ONE 7(2): e32523.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10318284
dc.description.abstractMembers of the genus Vibrio are important marine and aquaculture pathogens. Hemolytic activity has been identified as a virulence factor in many pathogenic vibrios including V. cholerae, V. parahaemolyticus, V. alginolyticus, V. harveyi and V. vulnificus. We have used transposon mutagenesis to identify genes involved in the hemolytic activity of shrimp-pathogenic V. harveyi strain PSU3316. Out of 1,764 mutants screened, five mutants showed reduced hemolytic activity on sheep blood agar and exhibited virulence attenuation in shrimp (Litopenaeus vannamei). Mutants were identified by comparing transposon junction sequences to a draft of assembly of the PSU3316 genome. Surprisingly none of the disrupted open reading frames or gene neighborhoods contained genes annotated as hemolysins. The gene encoding RseB, a negative regulator of the sigma factor (\(\sigma^E\)), was interrupted in 2 out of 5 transposon mutants, in addition, the transcription factor CytR, a threonine synthetase, and an efflux-associated cytoplasmic protein were also identified. Knockout mutations introduced into the rpoE operon at the rseB gene exhibited low hemolytic activity in sheep blood agar, and were 3-to 7-fold attenuated for colonization in shrimp. Comparison of whole cell extracted proteins in the rseB mutant (PSU4030) to the wild-type by 2-D gel electrophoresis revealed 6 differentially expressed proteins, including two down-regulated porins (OmpC-like and OmpN) and an upregulated protease (DegQ) which have been associated with \(\sigma^E\) in other organisms. Our study is the first report linking hemolytic activity to the \(\sigma^E\) regulators in pathogenic Vibrio species and suggests expression of this virulence-linked phenotype is governed by multiple regulatory pathways within the V. harveyi.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0032523en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285676/pdf/en_US
dash.licenseLAA
dc.subjectAgricultureen_US
dc.subjectAquacultureen_US
dc.subjectBiologyen_US
dc.subjectComputational Biologyen_US
dc.subjectMolecular Geneticsen_US
dc.subjectGeneticsen_US
dc.subjectGenetic Mutationen_US
dc.subjectImmunologyen_US
dc.subjectImmunityen_US
dc.subjectMicrobiologyen_US
dc.subjectVirologyen_US
dc.subjectProteomicsen_US
dc.titleSigma E Regulators Control Hemolytic Activity and Virulence in a Shrimp Pathogenic Vibrio harveyien_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorMekalanos, John J.
dc.date.available2013-02-21T18:27:33Z
dc.identifier.doi10.1371/journal.pone.0032523*
dash.contributor.affiliatedMekalanos, John


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