dc.contributor.author | Baum, Jennifer R. | |
dc.contributor.author | Dolmatova, Elena | |
dc.contributor.author | Tan, Alex Yu Hong | |
dc.contributor.author | Duffy, Heather S. | |
dc.date.accessioned | 2013-02-27T21:07:07Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Baum, Jennifer R., Elena Dolmatova, Alex Yu Hong Tan, and Heather S. Duffy. 2012. Omega 3 fatty acid inhibition of inflammatory cytokine-mediated Connexin43 regulation in the heart. Frontiers in Physiology 3:272. | en_US |
dc.identifier.issn | 1664-042X | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10352024 | |
dc.description.abstract | Background: The proinflammatory cytokine \(Interleukin-1\beta\) \((IL-1\beta)\), which increases in the heart post myocardial infarction (MI), has been shown to cause loss of Connexin43 (Cx43) function, an event known to underlie formation of the arrhythmogenic substrate. Omega 3 Fatty acids exhibit antiarrhythmic properties and impact \(IL-1\beta\) signaling. We hypothesize that Omega-3 fatty acids prevent arrhythmias in part, by inhibiting \(IL-1\beta\) signaling thus maintaining functional Cx43 channels. Methods: Rat neonatal myocytes or Madin-Darby Canine Kidney Epithelial (MDCK) cells grown in media in the absence (Ctr) or presence of \(30 \mu M\) docosahexaenoic acid (DHA, an Omega-3 Fatty acid) were treated with \(0.1 \mu M\) activated \(IL-1\beta\). We determined Cx43 channel function using a dye spread assay. Western blot and immunostaining were used to examine Cx43 levels/localization and downstream effectors of \(IL-1\beta\). In addition we used a murine model of MI for 24 h to determine the impact of an Omega-3 fatty acid enriched diet on Cx43 levels/localization post MI. Results: \(IL-1\beta\) significantly inhibited Cx43 function in Ctr cells \((200.9 \pm 17.7 \mu m [Ctr] vs. 112.8 \pm 14.9 \mu m [0.1 \mu M IL-1\beta], p<0.05)\). However, DHA-treated cells remained highly coupled in the presence of \(IL-1\beta\) \([167.9 \pm 21.9 \mu m [DHA] vs. 164.4 \pm 22.3 \mu m [DHA + 0.1 \ muM IL-1\beta], p<0.05, n = 4]\). Additionally, western blot showed that \(IL-1\beta\) treatment caused a 38.5% downregulation of Cx43 \([1.00 au [Ctr] vs. 0.615 au (0.1 \mu M IL-1\beta)\) which was completely abolished in DHA-treated cells \((0.935 au [DHA] vs. 1.02 au [DHA + 0.1 \mu M IL-1\beta), p < 0.05, n = 3]\). Examination of the downstream modulator of \(IL-1\beta\), \(NF\kappa \beta\) showed that while hypoxia caused translocation of \(NF\kappa \beta\) to the nucleus, this was inhibited by DHA. Additionally we found that a diet enriched in Omega-3 Fatty acids inhibited lateralization of Cx43 in the post-MI murine heart as well as limited activation of fibroblasts which would lead to decreased fibrosis overall. Conclusions: Omega 3 Fatty acid treatment inhibited \(IL-1\beta\)-stimulated loss of Cx43 protein, and more importantly, inhibited loss of Cx43 function by inhibiting translocation of \(NF\kappa \beta\). In the intact heart a diet enriched in Omega 3 Fatty Acids limited loss of Cx43 at the intercalated disk in the heart following MI. These data suggest that one of cardio-protective mechanisms by which Omega 3 Fatty acids work includes prevention of the pro-arrhythmic loss of Cx43 post MI and the attenuation of cardiac fibrosis after injury. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Frontiers | en_US |
dc.relation.isversionof | doi:10.3389/fphys.2012.00272 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429046 | en_US |
dash.license | LAA | |
dc.title | Omega 3 Fatty Acid Inhibition of Inflammatory Cytokine-Mediated Connexin43 Regulation in the Heart | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Frontiers in Physiology | en_US |
dash.depositing.author | Dolmatova, Elena | |
dc.date.available | 2013-02-27T21:07:07Z | |
dc.identifier.doi | 10.3389/fphys.2012.00272 | * |
dash.contributor.affiliated | Tan, Alex Yu Hong | |
dash.contributor.affiliated | Dolmatova, Elena | |