# Head-Group Conformation in Phospholipids: A Phosphorus-31 Nuclear Magnetic Resonance Study of Oriented Monodomain Dipalmitoylphosphatidylcholine Bilayers

 Title: Head-Group Conformation in Phospholipids: A Phosphorus-31 Nuclear Magnetic Resonance Study of Oriented Monodomain Dipalmitoylphosphatidylcholine Bilayers Author: Griffin, R. G.; Powers, L.; Pershan, Peter S. Note: Order does not necessarily reflect citation order of authors. Citation: Griffin, R. G., L. Powers, and Peter S. Pershan. 1978. Head-group conformation in phospholipids: A phosphorus-31 nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers. Biochemistry 17(14): 2718-2722. Full Text & Related Files: Griffin_head-group.pdf (1.504Mb; PDF) Abstract: Angular-dependent $$^{31}P$$ NMR spectra of oriented biaxial monodomain DPPG $$H_20$$ multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the 0-P-0 plane of the phosphate, where the 0’s are the nonesterified oxygens of the phosphodiester, is tilted at $$47 \pm 5^{\circ}$$ with respect to the bilayer normal. This $$PO_4$$ orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric $$^{31}P$$ powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems. Published Version: doi:10.1021/bi00607a004 Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10361972 Downloads of this work: