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dc.contributor.authorAsher, Sanford A.
dc.contributor.authorPershan, Peter S.
dc.date.accessioned2013-03-04T15:37:07Z
dc.date.issued1979
dc.identifier.citationAsher, Sanford A., and Peter S. Pershan. 1979. Alignment and defect structures in oriented phosphatidylcholine multilayers. Biophysical Journal 27(3): 393-421.en_US
dc.identifier.issn0006-3495en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10361979
dc.description.abstractThe alignment of dilauryl-, dimyristoyl-, and dipalmitoylphosphatidylcholine at various water concentrations into large oriented monodomain multilayers by annealing at elevated temperatures (Powers and Clark, 1975, Proc. Natl. Acad. Sci. U.S.A. 72:840; Powers and Pershan. 1977. Biophys. J. 20:137) is accompanied by the formation and subsequent dissolution of various defect structures. Some of these defects appear similar to those observed in thermotropic and other lyotropic liquid crystals, reflecting the lamellar structure of these materials. The formation and evolution of defects during the alignment of the lipids into the defect-free, monodomain, multilamellar geometry is studied using polarized microscopy. A combination of polarized and dark-field microscopy facilitated characterization of the defects; specific structural models are proposed. A new alignment technique involving compression and dilation of the lipid, which effects sample alignment at temperatures that are lower than those required by the Powers technique, is described. Lower temperature alignment avoids thermal decomposition that will sometimes occur if the lipid is maintained at elevated temperatures for prolonged periods. With this technique, samples \((80 \mu m thick)\) of dilaurylphosphatidylcholine with 20% water by weight were aligned at room temperature.en_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionofdoi:10.1016/S0006-3495(79)85225-Xen_US
dc.relation.hasversionhttp://www.pitt.edu/~asher/homepage/spec_pdf/Alignment%20and%20Defect%20Structures.pdfen_US
dash.licenseLAA
dc.titleAlignment and Defect Structures in Oriented Phosphatidylcholine Multilayersen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBiophysical Journalen_US
dash.depositing.authorPershan, Peter S.
dc.date.available2013-03-04T15:37:07Z
dc.identifier.doi10.1016/S0006-3495(79)85225-X*
dash.contributor.affiliatedPershan, Peter


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