Show simple item record

dc.contributor.authorSheridan, Steven D.
dc.contributor.authorSurampudi, Vasudha
dc.contributor.authorRao, Rajesh Raghavendran
dc.date.accessioned2013-03-04T15:37:59Z
dc.date.issued2012
dc.identifier.citationSheridan, Steven D., Vasudha Surampudi, and Raj R. Rao. 2012. Analysis of embryoid bodies derived from human induced pluripotent stem cells as a means to assess pluripotency. Stem Cells International 2012:738910.en_US
dc.identifier.issn1687-966Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10361980
dc.description.abstractHuman induced pluripotent stem cells (hiPSCs) have core properties of unlimited self-renewal and differentiation potential and have emerged as exciting cell sources for applications in regenerative medicine, drug discovery, understanding of development, and disease etiology. Key among numerous criteria to assess pluripotency includes the in vivo teratoma assay that has been widely proposed as a standard functional assay to demonstrate the pluripotency of hiPSCs. Yet, the lack of reliability across methodologies, lack of definitive clinical significance, and associated expenses bring into question use of the teratoma assay as the “gold standard” for determining pluripotency. We propose use of the in vitro embryoid body (EB) assay as an important alternative to the teratoma assay. This paper summarizes the methodologies for creating EBs from hiPSCs and the subsequent analyses to assess pluripotency and proposes its use as a cost-effective, controlled, and reproducible approach that can easily be adopted to determine pluripotency of generated hiPSCs.en_US
dc.language.isoen_USen_US
dc.publisherHindawi Publishing Corporationen_US
dc.relation.isversionofdoi:10.1155/2012/738910en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328185/pdf/en_US
dash.licenseLAA
dc.titleAnalysis of Embryoid Bodies Derived from Human Induced Pluripotent Stem Cells as a Means to Assess Pluripotencyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalStem Cells Internationalen_US
dash.depositing.authorRao, Rajesh Raghavendran
dc.date.available2013-03-04T15:37:59Z
dc.identifier.doi10.1155/2012/738910*
dash.contributor.affiliatedRao, Rajesh Raghavendran


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record