The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs

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The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs

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Title: The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs
Author: Christiansen, A. T.; Kiilgaard, J. F.; Ejstrup, R.; Wnek, G. E.; Prause, J. U.; Klassen, H.; Kaplan, H.; La Cour, M.; Smith, M.; Young, Michael Joseph

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Citation: Christiansen, A. T., J. F. Kiilgaard, M. Smith, R. Ejstrup, G. E. Wnek, J. U. Prause, M. J. Young, H. Klassen, H. Kaplan, and M. La Cour. 2012. The influence of brightness on functional assessment by mfERG: A study on scaffolds used in retinal cell transplantation in pigs. Stem Cells International 2012:263264.
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Abstract: To determine the effect of membrane brightness on multifocal electroretinograms (mfERGs), we implanted poly lactic-co-glycolic acid (PLGA) membranes in the subretinal space of 11 porcine eyes. We compared membranes with their native shiny white color with membranes that were stained with a blue dye (Brilliant Blue). Histological and electrophysiological evaluation of the overlying retina was carried out 6 weeks after implantation. Histologically, both white and blue membranes degraded in a spongiform manner leaving a disrupted outer retina with no preserved photoreceptor segments. Multifocal ERG revealed the white membranes to have a significantly higher P1-amplitude ratio than the blue (P = 0.027), and a correlation between brightness ratio and P1-amplitude ratio was found (r = 0.762). Based on our findings, we conclude that bright subretinal objects can produce normal mfERG amplitude ratios even when the adjacent photoreceptors are missing. Functional assessment with mfERG in scaffold implant studies should therefore be evaluated with care.
Published Version: doi:10.1155/2012/263264
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328220/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10361981
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