Targeted Parallel Sequencing of Large Genetically-Defined Genomic Regions for Identifying Mutations in Arabidopsis

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Targeted Parallel Sequencing of Large Genetically-Defined Genomic Regions for Identifying Mutations in Arabidopsis

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Title: Targeted Parallel Sequencing of Large Genetically-Defined Genomic Regions for Identifying Mutations in Arabidopsis
Author: Liu, Kun-Hsiang; McCormack, Matthew Augustus; Sheen, Jenq-Yunn

Note: Order does not necessarily reflect citation order of authors.

Citation: Liu, Kun-hsiang, Matthew McCormack, and Jen Sheen. 2012. Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis. Plant Methods 8:12.
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Abstract: Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal recombination. Current strategies using next generation sequencing for molecular identification of mutations require whole genome sequencing and advanced computational devises and skills, which are not readily accessible or affordable to every laboratory. We have developed a streamlined method using parallel massive sequencing for mutant identification in which only targeted regions are sequenced. This targeted parallel sequencing (TPSeq) method is more cost-effective, straightforward enough to be easily done without specialized bioinformatics expertise, and reliable for identifying multiple mutations simultaneously. Here, we demonstrate its use by identifying three novel nitrate-signaling mutants in Arabidopsis.
Published Version: doi:10.1186/1746-4811-8-12
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3348062/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10378098
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