dc.contributor.author | Li, Gene-Wei | |
dc.contributor.author | Xie, Xiaoliang Sunney | |
dc.date.accessioned | 2013-03-12T19:11:45Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Li, Gene-Wei and X. Sunney Xie. 2012. Central dogma at the single-molecule level in living cells. Nature 475(7356): 308-315. | en_US |
dc.identifier.issn | 0028-0836 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10386976 | |
dc.description.abstract | Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time. This leads to heterogeneity in the messenger RNA and protein copy numbers in a population of cells with identical genomes. With advanced single-cell fluorescence microscopy, it is now possible to quantify transcriptomes and proteomes with single-molecule sensitivity. Dynamic processes such as transcription factor binding, transcription and translation can be monitored in real time, providing quantitative descriptions of gene expression and regulation, and the demonstration that a single-molecule event can determine the phenotype of a cell. | en_US |
dc.description.sponsorship | Chemistry and Chemical Biology | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | doi:10.1038/nature10315 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pubmed/21776076 | en_US |
dash.license | OAP | |
dc.title | Central Dogma at the Single-Molecule Level in Living Cells | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Nature | en_US |
dash.depositing.author | Xie, Xiaoliang Sunney | |
dc.date.available | 2013-03-12T19:11:45Z | |
dc.identifier.doi | 10.1038/nature10315 | * |
dash.contributor.affiliated | Xie, Xiaoliang | |