Video Rate Molecular Imaging In Vivo with Stimulated Raman Scattering

DSpace/Manakin Repository

Video Rate Molecular Imaging In Vivo with Stimulated Raman Scattering

Citable link to this page

 

 
Title: Video Rate Molecular Imaging In Vivo with Stimulated Raman Scattering
Author: Saar, Brian; Freudiger, Christian Wilhelm; Reichman, Jay; Stanley, C. Michael; Holtom, Gary; Xie, Xiaoliang Sunney

Note: Order does not necessarily reflect citation order of authors.

Citation: Saar, Brian G., Christian W. Freudiger, Jay Reichman, C. Michael Stanley, Gary R. Holtom, and X. Sunney Xie. 2010. Video-rate molecular imaging in vivo with simulated Raman scattering. Science 330(6009): 1368-1370.
Full Text & Related Files:
Abstract: Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not been feasible because light cannot be collected through thick tissues, and motion-blur arises from slow imaging based on backscattered light. In this work, we enable in vivo SRS imaging by substantially enhancing the collection of the backscattered signal and increasing the imaging speed by three orders of magnitude to video rate. This approach allows label-free in vivo imaging of water, lipid, and protein in skin and mapping of penetration pathways of topically applied drugs in mice and humans.
Published Version: doi:10.1126/science.1197236
Other Sources: http://bernstein.harvard.edu/papers/Saar%20Science%2012.3.2010.pdf
http://web.mit.edu/hst.035/ReadingSupplement/05_03_Xie/Saar%20Science%2012.3.2010.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10399823
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters