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dc.contributor.authorJanic, Branislava
dc.contributor.authorJafari-Khouzani, Kourosh
dc.contributor.authorBabajani-Feremi, Abbas
dc.contributor.authorIskander, A. S. M.
dc.contributor.authorVarma, Nadimpalli Ravi S.
dc.contributor.authorAli, Meser M.
dc.contributor.authorKnight, Robert A.
dc.contributor.authorArbab, Ali S.
dc.date.accessioned2013-03-14T18:36:45Z
dc.date.issued2012
dc.identifier.citationJanic, Branislava, Kourosh Jafari-Khouzani, Abbas Babajani-Feremi, A. S. M. Iskander, Nadimpalli Ravi S. Varma, Meser M. Ali, Robert A. Knight, and Ali S. Arbab. 2012. MRI tracking of FePro labeled fresh and cryopreserved long term in vitro expanded human cord blood AC133+ endothelial progenitor cells in rat glioma. PLoS ONE 7(5): e37577.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10406322
dc.description.abstractBackground: Endothelial progenitors cells (EPCs) are important for the development of cell therapies for various diseases. However, the major obstacles in developing such therapies are low quantities of EPCs that can be generated from the patient and the lack of adequate non-invasive imaging approach for in vivo monitoring of transplanted cells. The objective of this project was to determine the ability of cord blood (CB) AC133+ EPCs to differentiate, in vitro and in vivo, toward mature endothelial cells (ECs) after long term in vitro expansion and cryopreservation and to use magnetic resonance imaging (MRI) to assess the in vivo migratory potential of ex vivo expanded and cryopreserved CB AC133+ EPCs in an orthotopic glioma rat model. Materials, Methods and Results: The primary CB AC133+ EPC culture contained mainly EPCs and long term in vitro conditions facilitated the maintenance of these cells in a state of commitment toward endothelial lineage. At days 15–20 and 25–30 of the primary culture, the cells were labeled with FePro and cryopreserved for a few weeks. Cryopreserved cells were thawed and in vitro differentiated or IV administered to glioma bearing rats. Different groups of rats also received long-term cultured, magnetically labeled fresh EPCs and both groups of animals underwent MRI 7 days after IV administration of EPCs. Fluorescent microscopy showed that in vitro differentiation of EPCs was not affected by FePro labeling and cryopreservation. MRI analysis demonstrated that in vivo accumulation of previously cryopreserved transplanted cells resulted in significantly higher R2 and R2* values indicating a higher rate of migration and incorporation into tumor neovascularization of previously cryopreserved CB AC133+ EPCs to glioma sites, compared to non-cryopreserved cells. Conclusion: Magnetically labeled CB EPCs can be in vitro expanded and cryopreserved for future use as MRI probes for monitoring the migration and incorporation to the sites of neovascularization.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0037577en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360770/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Typesen_US
dc.subjectStem Cellsen_US
dc.subjectAdult Stem Cellsen_US
dc.subjectMedicineen_US
dc.subjectRadiologyen_US
dc.subjectDiagnostic Radiologyen_US
dc.subjectMagnetic Resonance Imagingen_US
dc.titleMRI Tracking of FePro Labeled Fresh and Cryopreserved Long Term In Vitro Expanded Human Cord Blood AC133+ Endothelial Progenitor Cells in Rat Gliomaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorJafari-Khouzani, Kourosh
dc.date.available2013-03-14T18:36:45Z
dc.identifier.doi10.1371/journal.pone.0037577*
dash.contributor.affiliatedJafari-Khouzani, Kourosh


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