MRI Tracking of FePro Labeled Fresh and Cryopreserved Long Term In Vitro Expanded Human Cord Blood AC133+ Endothelial Progenitor Cells in Rat Glioma

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MRI Tracking of FePro Labeled Fresh and Cryopreserved Long Term In Vitro Expanded Human Cord Blood AC133+ Endothelial Progenitor Cells in Rat Glioma

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dc.contributor.author Janic, Branislava
dc.contributor.author Babajani-Feremi, Abbas
dc.contributor.author Iskander, A. S. M.
dc.contributor.author Varma, Nadimpalli Ravi S.
dc.contributor.author Ali, Meser M.
dc.contributor.author Knight, Robert A.
dc.contributor.author Arbab, Ali S.
dc.contributor.author Jafari-Khouzani, Kourosh
dc.date.accessioned 2013-03-14T18:36:45Z
dc.date.issued 2012
dc.identifier.citation Janic, Branislava, Kourosh Jafari-Khouzani, Abbas Babajani-Feremi, A. S. M. Iskander, Nadimpalli Ravi S. Varma, Meser M. Ali, Robert A. Knight, and Ali S. Arbab. 2012. MRI tracking of FePro labeled fresh and cryopreserved long term in vitro expanded human cord blood AC133+ endothelial progenitor cells in rat glioma. PLoS ONE 7(5): e37577. en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:10406322
dc.description.abstract Background: Endothelial progenitors cells (EPCs) are important for the development of cell therapies for various diseases. However, the major obstacles in developing such therapies are low quantities of EPCs that can be generated from the patient and the lack of adequate non-invasive imaging approach for in vivo monitoring of transplanted cells. The objective of this project was to determine the ability of cord blood (CB) AC133+ EPCs to differentiate, in vitro and in vivo, toward mature endothelial cells (ECs) after long term in vitro expansion and cryopreservation and to use magnetic resonance imaging (MRI) to assess the in vivo migratory potential of ex vivo expanded and cryopreserved CB AC133+ EPCs in an orthotopic glioma rat model. Materials, Methods and Results: The primary CB AC133+ EPC culture contained mainly EPCs and long term in vitro conditions facilitated the maintenance of these cells in a state of commitment toward endothelial lineage. At days 15–20 and 25–30 of the primary culture, the cells were labeled with FePro and cryopreserved for a few weeks. Cryopreserved cells were thawed and in vitro differentiated or IV administered to glioma bearing rats. Different groups of rats also received long-term cultured, magnetically labeled fresh EPCs and both groups of animals underwent MRI 7 days after IV administration of EPCs. Fluorescent microscopy showed that in vitro differentiation of EPCs was not affected by FePro labeling and cryopreservation. MRI analysis demonstrated that in vivo accumulation of previously cryopreserved transplanted cells resulted in significantly higher R2 and R2* values indicating a higher rate of migration and incorporation into tumor neovascularization of previously cryopreserved CB AC133+ EPCs to glioma sites, compared to non-cryopreserved cells. Conclusion: Magnetically labeled CB EPCs can be in vitro expanded and cryopreserved for future use as MRI probes for monitoring the migration and incorporation to the sites of neovascularization. en_US
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.isversionof doi:10.1371/journal.pone.0037577 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360770/pdf/ en_US
dash.license LAA
dc.subject Biology en_US
dc.subject Molecular Cell Biology en_US
dc.subject Cellular Types en_US
dc.subject Stem Cells en_US
dc.subject Adult Stem Cells en_US
dc.subject Medicine en_US
dc.subject Radiology en_US
dc.subject Diagnostic Radiology en_US
dc.subject Magnetic Resonance Imaging en_US
dc.title MRI Tracking of FePro Labeled Fresh and Cryopreserved Long Term In Vitro Expanded Human Cord Blood AC133+ Endothelial Progenitor Cells in Rat Glioma en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal PLoS ONE en_US
dash.depositing.author Jafari-Khouzani, Kourosh
dc.date.available 2013-03-14T18:36:45Z

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