Live Imaging of Mitosomes and Hydrogenosomes by HaloTag Technology

 
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Martincová, Eva
Voleman, Luboš
Najdrová, Vladimíra
De Napoli, Maximiliano
Eshar, Shiri
Gualdron, Melisa
Hopp, Christine S.
Sanin, David E.
Tembo, Dumizulu L.
Walker, Dawn
Marcinčiková, Michaela
Tachezy, Jan
Doležal, Pavel
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1371/journal.pone.0036314
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Martincová, Eva, Luboš Voleman, Vladimíra Najdrová, Maximiliano De Napoli, Shiri Eshar, Melisa Gualdron, Christine S. Hopp, et al. 2012. Live imaging of mitosomes and hydrogenosomes by halotag technology. PLoS ONE 7(4): e36314.
Abstract
Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338651/pdf/
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This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:10433474

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