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dc.contributor.authorMartincová, Eva
dc.contributor.authorVoleman, Luboš
dc.contributor.authorNajdrová, Vladimíra
dc.contributor.authorDe Napoli, Maximiliano
dc.contributor.authorEshar, Shiri
dc.contributor.authorGualdron, Melisa
dc.contributor.authorHopp, Christine S.
dc.contributor.authorSanin, David E.
dc.contributor.authorTembo, Dumizulu L.
dc.contributor.authorWalker, Dawn
dc.contributor.authorMarcinčiková, Michaela
dc.contributor.authorTachezy, Jan
dc.contributor.authorDoležal, Pavel
dc.contributor.authorVan Tyne, Daria Natalie
dc.date.accessioned2013-03-18T16:53:34Z
dc.date.issued2012
dc.identifier.citationMartincová, Eva, Luboš Voleman, Vladimíra Najdrová, Maximiliano De Napoli, Shiri Eshar, Melisa Gualdron, Christine S. Hopp, et al. 2012. Live imaging of mitosomes and hydrogenosomes by halotag technology. PLoS ONE 7(4): e36314.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10433474
dc.description.abstractHydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0036314en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338651/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectCytochemistryen_US
dc.subjectOrganellesen_US
dc.subjectEvolutionary Biologyen_US
dc.subjectOrganismal Evolutionen_US
dc.subjectEukaryotic Evolutionen_US
dc.subjectMicrobiologyen_US
dc.subjectParasitologyen_US
dc.subjectParasite Evolutionen_US
dc.subjectProtozoologyen_US
dc.subjectParastic Protozoansen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Structuresen_US
dc.subjectSubcellular Organellesen_US
dc.titleLive Imaging of Mitosomes and Hydrogenosomes by HaloTag Technologyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorVan Tyne, Daria Natalie
dc.date.available2013-03-18T16:53:34Z
dc.identifier.doi10.1371/journal.pone.0036314*
dash.authorsorderedfalse
dash.contributor.affiliatedVan tyne, Daria


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