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dc.contributor.authorHong, Sunhee
dc.contributor.authorCheng, Tan-Yun
dc.contributor.authorLayre, Emilie
dc.contributor.authorSweet, Lindsay
dc.contributor.authorYoung, David C.
dc.contributor.authorPosey, James E.
dc.contributor.authorButler, W. Ray
dc.contributor.authorMoody, D Branch
dc.date.accessioned2013-03-18T17:05:38Z
dc.date.issued2012
dc.identifier.citationHong, Sunhee, Tan-Yun Cheng, Emilie Layre, Lindsay Sweet, David C. Young, James E. Posey, W. Ray Butler, and D. Branch Moody. 2012. Ultralong C100 mycolic acids support the assignment of segniliparus as a new bacterial genus. PLoS ONE 7(6).en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10433481
dc.description.abstractMycolic acid-producing bacteria isolated from the respiratory tract of human and non-human mammals were recently assigned as a distinct genus, Segniliparus, because they diverge from rhodococci and mycobacteria in genetic and chemical features. Using high accuracy mass spectrometry, we determined the chemical composition of 65 homologous mycolic acids in two Segniliparus species and separately analyzed the three subclasses to measure relative chain length, number and stereochemistry of unsaturations and cyclopropyl groups within each class. Whereas mycobacterial mycolate subclasses are distinguished from one another by R groups on the meromycolate chain, Segniliparus species synthesize solely non-oxygenated α-mycolates with high levels of cis unsaturation. Unexpectedly Segniliparus α-mycolates diverge into three subclasses based on large differences in carbon chain length with one bacterial culture producing mycolates that range from C58 to C100. Both the overall chain length (C100) and the chain length diversity (C42) are larger than previously seen for mycolic acid-producing organisms and provide direct chemical evidence for assignment of Segniliparus as a distinct genus. Yet, electron microscopy shows that the long and diverse mycolates pack into a typical appearing membrane. Therefore, these new and unexpected extremes of mycolic acid chemical structure raise questions about the modes of mycolic acid packing and folding into a membrane.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0039017en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375245/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectLipidsen_US
dc.subjectLipid Structureen_US
dc.subjectEvolutionary Biologyen_US
dc.subjectEvolutionary Systematicsen_US
dc.subjectTaxonomyen_US
dc.subjectMicrobial Taxonomyen_US
dc.subjectMicrobiologyen_US
dc.subjectBacteriologyen_US
dc.subjectBacterial Taxonomyen_US
dc.subjectMedicineen_US
dc.subjectDiagnostic Medicineen_US
dc.subjectPathologyen_US
dc.subjectClinical Pathologyen_US
dc.subjectClinical Microbiologyen_US
dc.titleUltralong C100 Mycolic Acids Support the Assignment of Segniliparus as a New Bacterial Genusen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorMoody, D Branch
dc.date.available2013-03-18T17:05:38Z
dc.identifier.doi10.1371/journal.pone.0039017*
dash.contributor.affiliatedLayre, Emilie
dash.contributor.affiliatedMoody, David


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