MicroRNAs Are Indispensable for Reprogramming Mouse Embryonic Fibroblasts into Induced Stem Cell-Like Cells

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MicroRNAs Are Indispensable for Reprogramming Mouse Embryonic Fibroblasts into Induced Stem Cell-Like Cells

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Title: MicroRNAs Are Indispensable for Reprogramming Mouse Embryonic Fibroblasts into Induced Stem Cell-Like Cells
Author: Thier, Marc-Christian; Oh, Sangnam; Kanellopoulou, Chryssa; Edenhofer, Frank; Kim, Byeong-Moo; Sherwood, Richard Irving; Choi, Michael Yoon-Suk

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Citation: Kim, Byeong-Moo, Marc-Christian Thier, Sangnam Oh, Richard Sherwood, Chryssa Kanellopoulou, Frank Edenhofer, and Michael Y. Choi. 2012. Micrornas are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. PLoS ONE 7(6): e39239.
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Abstract: MicroRNAs play a pivotal role in cellular maintenance, proliferation, and differentiation. They have also been implicated to play a key role in disease pathogenesis, and more recently, cellular reprogramming. Certain microRNA clusters can enhance or even directly induce reprogramming, while repressing key proteins involved in microRNA processing decreases reprogramming efficiency. Although microRNAs clearly play important roles in cellular reprogramming, it remains unknown whether microRNAs are absolutely necessary. We endeavored to answer this fundamental question by attempting to reprogram Dicer-null mouse embryonic fibroblasts (MEFs) that lack almost all functional microRNAs using a defined set of transcription factors. Transduction of reprogramming factors using either lentiviral or piggyBac transposon vector into two, independently derived lines of Dicer-null MEFs failed to produce cells resembling embryonic stem cells (ESCs). However, expression of human Dicer in the Dicer-null MEFs restored their reprogramming potential. Our study demonstrates for the first time that microRNAs are indispensable for dedifferentiation reprogramming.
Published Version: doi:10.1371/journal.pone.0039239
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380844/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10436249
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