Stimulation of Host Immune Defenses by a Small Molecule Protects C. elegans from Bacterial Infection

DSpace/Manakin Repository

Stimulation of Host Immune Defenses by a Small Molecule Protects C. elegans from Bacterial Infection

Citable link to this page

 

 
Title: Stimulation of Host Immune Defenses by a Small Molecule Protects C. elegans from Bacterial Infection
Author: Larkins-Ford, Jonah; Conery, Annie L.; Pukkila-Worley, Read; Feinbaum, Rhonda Lorin; Kirienko, Natalia; Ausubel, Frederick M.

Note: Order does not necessarily reflect citation order of authors.

Citation: Pukkila-Worley, Read, Rhonda Feinbaum, Natalia V. Kirienko, Jonah Larkins-Ford, Annie L. Conery, and Frederick M. Ausubel. 2012. Stimulation of host immune defenses by a small molecule protects C. elegans from bacterial infection. PLoS Genetics 8(6): e1002733.
Full Text & Related Files:
Abstract: The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular processes. We previously used a bacterial infection assay in C. elegans to identify 119 compounds that affect host-microbe interactions among 37,214 tested. Here we show that one of these small molecules, RPW-24, protects C. elegans from bacterial infection by stimulating the host immune response of the nematode. Using transcriptome profiling, epistasis pathway analyses with C. elegans mutants, and an RNAi screen, we show that RPW-24 promotes resistance to Pseudomonas aeruginosa infection by inducing the transcription of a remarkably small number of C. elegans genes (\(\sim\)1.3% of all genes) in a manner that partially depends on the evolutionarily-conserved p38 MAP kinase pathway and the transcription factor ATF-7. These data show that the immunostimulatory activity of RPW-24 is required for its efficacy and define a novel C. elegans–based strategy to identify compounds with activity against antibiotic-resistant bacterial pathogens.
Published Version: doi:10.1371/journal.pgen.1002733
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375230/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10436284
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters