miR-1289 and “Zipcode”-like Sequence Enrich mRNAs in Microvesicles

 
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Bolukbasi, Mehmet Fatih
Mizrak, Arda
Ozdener, Gokhan Baris
Madlener, Sibylle
Ströbel, Thomas
Erkan, Erdogan Pekcan
Fan, Jian-Bing
Saydam, Okay
Published Version
https://doi.org/10.1038/mtna.2011.2
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Bolukbasi, Mehmet Fatih, Arda Mizrak, Gokhan Baris Ozdener, Sibylle Madlener, Thomas Ströbel, Erdogan Pekcan Erkan, Jian-Bing Fan, Xandra O Breakefield, and Okay Saydam. 2012. miR-1289 and “zipcode”-like sequence enrich mRNSs in microvesicles. Molecular Therapy Nucleic Acids 1(2): e10.
Abstract
Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in the 3′-untranslated region (3′UTR) of mRNAs, with variants of this sequence present in many mRNAs enriched in MVs, as compared to their glioblastoma cells of origin. When this sequence was incorporated into the 3′UTR of a reporter message and expressed in a different cell type, it led to enrichment of the reporter mRNA in MVs. Critical features of this sequence are both a CUGCC core presented on a stem-loop structure and a miRNA-binding site, with increased levels of the corresponding miRNA in cells further increasing levels of mRNAs in MVs.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3381601/pdf/
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:10445570

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