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dc.contributor.authorSholukh, Anton M.
dc.contributor.authorMukhtar, Muhammad Mahmood
dc.contributor.authorHumbert, Michael
dc.contributor.authorEssono, Sosthène S.
dc.contributor.authorWatkins, Jennifer D.
dc.contributor.authorVyas, Hemant Kumar
dc.contributor.authorShanmuganathan, Vivekanandan
dc.contributor.authorHemashettar, Girish
dc.contributor.authorKahn, Maria
dc.contributor.authorHu, Shiu-Lok
dc.contributor.authorMontefiori, David C.
dc.contributor.authorPolonis, Victoria R.
dc.contributor.authorSchur, Peter Henry
dc.contributor.authorRuprecht, Ruth Margrit
dc.date.accessioned2013-03-19T17:52:45Z
dc.date.issued2012
dc.identifier.citationSholukh, Anton M., Muhammad M. Mukhtar, Michael Humbert, Sosthène S. Essono, Jennifer D. Watkins, Hemant K. Vyas, Vivekanandan Shanmuganathan, Girish Hemashettar, Maria Kahn, Shiu-Lok Hu, David C. Montefiori, Victoria R. Polonis, Peter H. Schur, and Ruth M. Ruprecht. 2012. Isolation of monoclonal antibodies with predetermined conformational epitope specificity. PLoS ONE 7(6): e38943.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10445601
dc.description.abstractExisting technologies allow isolating antigen-specific monoclonal antibodies (mAbs) from B cells. We devised a direct approach to isolate mAbs with predetermined conformational epitope specificity, using epitope mimetics (mimotopes) that reflect the three-dimensional structure of given antigen subdomains. We performed differential biopanning using bacteriophages encoding random peptide libraries and polyclonal antibodies (Abs) that had been affinity-purified with either native or denatured antigen. This strategy yielded conformational mimotopes. We then generated mimotope-fluorescent protein fusions, which were used as baits to isolate single memory B cells from rhesus monkeys (RMs). To amplify RM immunoglobulin variable regions, we developed RM-specific PCR primers and generated chimeric simian-human mAbs with predicted epitope specificity. We established proof-of-concept of our strategy by isolating mAbs targeting the conformational V3 loop crown of HIV Env; the new mAbs cross-neutralized viruses of different clades. The novel technology allows isolating mAbs from RMs or other hosts given experimental immunogens or infectious agents.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0038943en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3380854/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiotechnologyen_US
dc.subjectBioengineeringen_US
dc.subjectImmunologyen_US
dc.subjectImmune Cellsen_US
dc.subjectB Cellsen_US
dc.subjectImmunityen_US
dc.subjectHumoral Immunityen_US
dc.subjectImmune Responseen_US
dc.subjectImmunoglobulinsen_US
dc.subjectImmunologic Techniquesen_US
dc.subjectMicrobiologyen_US
dc.subjectVirologyen_US
dc.subjectViral Structureen_US
dc.subjectViral Envelopeen_US
dc.subjectImmunodeficiency Virusesen_US
dc.subjectMechanisms of Resistance and Susceptibilityen_US
dc.subjectViral Vaccinesen_US
dc.subjectMedicineen_US
dc.subjectInfectious Diseasesen_US
dc.subjectSexually Transmitted Diseasesen_US
dc.subjectAIDSen_US
dc.subjectViral Diseasesen_US
dc.subjectHIVen_US
dc.subjectRetrovirology and HIV immunopathogenesisen_US
dc.titleIsolation of Monoclonal Antibodies with Predetermined Conformational Epitope Specificityen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorSchur, Peter Henry
dc.date.available2013-03-19T17:52:45Z
dc.identifier.doi10.1371/journal.pone.0038943*
dash.contributor.affiliatedSchur, Peter
dash.contributor.affiliatedMukhtar, Muhammad Mahmood
dash.contributor.affiliatedVyas, Hemant Kumar
dash.contributor.affiliatedWatkins, Jennifer D.
dash.contributor.affiliatedSholukh, Anton M.
dash.contributor.affiliatedHumbert, Michael
dash.contributor.affiliatedRuprecht, Ruth Margrit


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