Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

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Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

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Title: Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
Author: Carr, Peter A.; Isaacs, Farren J.; Wang, Harris He; Sterling, Bram; Lajoie, Marc Joseph; Xu, George Jing; Church, George McDonald; Jacobson, Joseph M.

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Citation: Carr, Peter A., Harris H. Wang, Bram Sterling, Farren J. Isaacs, Marc J. Lajoie, George Xu, George M. Church, and Joseph M. Jacobson. 2012. Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection. Nucleic Acids Research 40(17): e132.
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Abstract: Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome engineering that yields highly modified cells. We demonstrate that disparate sites throughout the genome can be easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites. We apply this technique to the modification of 80 sites in the E. coli genome.
Published Version: doi:10.1093/nar/gks455
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458525/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10445643
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