Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy

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Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy

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Title: Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy
Author: Horton, Nicholas G.; Wang, Ke; Liu, Tzu-Ming; Wu, Juwell; Lin, Charles P.; Xu, Chris

Note: Order does not necessarily reflect citation order of authors.

Citation: Wang, Ke, Tzu-Ming Liu, Juwell Wu, Nicholas G. Horton, Charles P. Lin, and Chris Xu. 2012. Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy. Biomedical Optics Express 3(9): 1972-1977.
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Abstract: We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a “rainbow” combination of these three fluorescent proteins.
Published Version: doi:10.1364/BOE.3.001972
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447541/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10448706
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