Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy
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Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy
| Title: | Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy |
| Author: |
Horton, Nicholas G.; Wang, Ke; Liu, Tzu-Ming; Wu, Juwell; Lin, Charles P.; Xu, Chris
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Wang, Ke, Tzu-Ming Liu, Juwell Wu, Nicholas G. Horton, Charles P. Lin, and Chris Xu. 2012. Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy. Biomedical Optics Express 3(9): 1972-1977. |
| Full Text & Related Files: |
3447541.pdf (2.784Mb; PDF) 
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| Abstract: | We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a “rainbow” combination of these three fluorescent proteins. |
| Published Version: | doi:10.1364/BOE.3.001972 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447541/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10448706 |
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